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Pulse Field Gel Electrophoresis

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Kieran Jordan

    1. Teagasc Food Research Center, Moorepark, Fermoy, Co. Cork, Ireland

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    You can also search for this editor in PubMed   Google Scholar

  2. Marion Dalmasso

    1. Department of Microbiology & Alimentary Pharmabiotic Centre, University College Cork, Co. Cork, Ireland

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step details essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1301)

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Table of contents (21 protocols)

  1. PFGE and Non-pathogenic Bacteria

    1. PFGE Protocols to Distinguish Subspecies of Lactococcus lactis

      • Pascal Le Bourgeois, Delphine Passerini, Michèle Coddeville, Maéva Guellerin, Marie-Line Daveran-Mingot, Paul Ritzenthaler
      Pages 213-224

    2. The Use of PFGE Method in Genotyping of Selected Bacteria Species of the Lactobacillus Genus

      • Tomasz Gosiewski, Monika Brzychczy-Wloch
      Pages 225-240

    3. Pulsed-Field Gel Electrophoresis for Leuconostoc mesenteroides and L. pseudomesenteroides

      • Victoria Chuat, Marion Dalmasso
      Pages 241-251

    4. Pulsed Field Gel Electrophoresis for Bifidobacterium

      • Esther Jiménez, Marta Gómez, Laura Moles
      Pages 253-263

    5. Pulsed Field Gel Electrophoresis for Dairy Propionibacteria

      • Victoria Chuat, Rosangela de Freitas, Marion Dalmasso
      Pages 265-275

  2. Back Matter

    Pages 277-278
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Keywords

About this book

This  volume will be of interest to epidemiologists, food microbiologists, and anyone working on comparing bacterial isolates. Pulse Field Gel Electrophoresis: Methods and Protocols guides readers through methods and protocols that will advance the harmonisation of PFGE methodologies and facilitate inter-laboratory comparisons of PFGE profiles from pathogenic and non-pathogenic bacteria. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

Concise and easy-to-use, Pulse Field Gel Electrophoresis: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Teagasc Food Research Center, Moorepark, Fermoy, Co. Cork, Ireland

    Kieran Jordan

  • Department of Microbiology & Alimentary Pharmabiotic Centre, University College Cork, Co. Cork, Ireland

    Marion Dalmasso

Bibliographic Information

  • Book Title: Pulse Field Gel Electrophoresis

  • Book Subtitle: Methods and Protocols

  • Editors: Kieran Jordan, Marion Dalmasso

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2599-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2598-8Published: 11 April 2015

  • Softcover ISBN: 978-1-4939-5588-6Published: 05 October 2016

  • eBook ISBN: 978-1-4939-2599-5Published: 10 April 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 278

  • Number of Illustrations: 24 b/w illustrations, 20 illustrations in colour

  • Topics: Microbiology

  • Industry Sectors: Biotechnology, Chemical Manufacturing, Consumer Packaged Goods, Pharma

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