Abstract
Pulsed-field gel electrophoresis (PFGE), developed in the mid-1980s, rapidly became a “gold standard” method for analyzing bacterial chromosomes. Today, although outcompeted in resolution by alternative methods, such as optical mapping, and not applicable for high-throughput analyses, PFGE remains a valuable method for bacterial strain typing. Here, we describe optimized protocols for macrorestriction fingerprinting, characterization of plasmid complements, and gene localization by DNA-DNA hybridization of Lactococcus lactis genomes.
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Acknowledgments
This work was supported by the Centre National de la Recherche Scientifique, the University Toulouse III–University Paul Sabatier, and the Région Midi-Pyrénées (APRRTT 2010 program).
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Le Bourgeois, P., Passerini, D., Coddeville, M., Guellerin, M., Daveran-Mingot, ML., Ritzenthaler, P. (2015). PFGE Protocols to Distinguish Subspecies of Lactococcus lactis . In: Jordan, K., Dalmasso, M. (eds) Pulse Field Gel Electrophoresis. Methods in Molecular Biology, vol 1301. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2599-5_17
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DOI: https://doi.org/10.1007/978-1-4939-2599-5_17
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