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Pulse Field Gel Electrophoresis

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Kieran Jordan

    1. Teagasc Food Research Center, Moorepark, Fermoy, Co. Cork, Ireland

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    You can also search for this editor in PubMed   Google Scholar

  2. Marion Dalmasso

    1. Department of Microbiology & Alimentary Pharmabiotic Centre, University College Cork, Co. Cork, Ireland

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step details essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1301)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
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  3. PFGE and Pathogenic Bacteria

    1. Front Matter

      Pages 41-41
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    2. Pulsed-Field Gel Electrophoresis for Listeria monocytogenes

      • Laura Luque-Sastre, Séamus Fanning, Edward M. Fox
      Pages 43-53

    3. Pulsed-Field Gel Electrophoresis (PFGE) for Pathogenic Cronobacter Species

      • Qiongqiong Yan, Séamus Fanning
      Pages 55-69

    4. Pulsed-Field Gel Electrophoresis of Bacillus cereus Group Strains

      • Paul Drean, Edward M. Fox
      Pages 71-83

    5. Pulsed-Field Gel Electrophoresis of Staphylococcus aureus

      • George R. Golding, Jennifer Campbell, Dave Spreitzer, Linda Chui
      Pages 85-93

    6. The Use of Pulsed-Field Gel Electrophoresis for Genotyping of Clostridium difficile

      • Wondwossen A. Gebreyes, Pamela R. F. Adkins
      Pages 95-101

    7. Molecular Subtyping of Clostridium botulinum by Pulsed-Field Gel Electrophoresis

      • Carolina Lúquez, Lavin A. Joseph, Susan E. Maslanka
      Pages 103-113

    8. Pulsed-Field Gel Electrophoresis of Yersinia pestis

      • Tamara Revazishvili, Judith A. Johnson
      Pages 115-128

    9. Pulsed Field Gel Electrophoresis of Group A Streptococci

      • Luca Agostino Vitali, Giovanni Gherardi, Dezemona Petrelli
      Pages 129-138

    10. Application of Pulsed Field Gel Electrophoresis to Type Campylobacter jejuni

      • Ping Zhou, Omar A. Oyarzabal
      Pages 139-156

    11. Pulsed-Field Gel Electrophoresis of Pseudomonas aeruginosa

      • Honghua Hu, Jim Manos
      Pages 157-170

    12. PFGE for Shiga Toxin-Producing Escherichia coli O157:H7 (STEC O157) and Non-O157 STEC

      • Patricia Jaros, Muriel Dufour, Brent Gilpin, Molly M. Freeman, Efrain M. Ribot
      Pages 171-189

    13. Pulsed-Field Gel Electrophoresis of Salmonella enterica

      • Antonio Camarda, Elena Circella, Antonia Pupillo, Marilisa Legretto, Michele Marino, Nicola Pugliese
      Pages 191-210

  4. PFGE and Non-pathogenic Bacteria

    1. Front Matter

      Pages 211-211
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Keywords

About this book

This  volume will be of interest to epidemiologists, food microbiologists, and anyone working on comparing bacterial isolates. Pulse Field Gel Electrophoresis: Methods and Protocols guides readers through methods and protocols that will advance the harmonisation of PFGE methodologies and facilitate inter-laboratory comparisons of PFGE profiles from pathogenic and non-pathogenic bacteria. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

Concise and easy-to-use, Pulse Field Gel Electrophoresis: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Teagasc Food Research Center, Moorepark, Fermoy, Co. Cork, Ireland

    Kieran Jordan

  • Department of Microbiology & Alimentary Pharmabiotic Centre, University College Cork, Co. Cork, Ireland

    Marion Dalmasso

Bibliographic Information

  • Book Title: Pulse Field Gel Electrophoresis

  • Book Subtitle: Methods and Protocols

  • Editors: Kieran Jordan, Marion Dalmasso

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2599-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2598-8Published: 11 April 2015

  • Softcover ISBN: 978-1-4939-5588-6Published: 05 October 2016

  • eBook ISBN: 978-1-4939-2599-5Published: 10 April 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 278

  • Number of Illustrations: 24 b/w illustrations, 20 illustrations in colour

  • Topics: Microbiology

  • Industry Sectors: Biotechnology, Chemical Manufacturing, Consumer Packaged Goods, Pharma

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