Abstract
Pulsed field gel electrophoresis (PFGE) is a technique using alternating electric fields to migrate high molecular weight DNA fragments with a high resolution. This method consists of the digestion of bacterial chromosomal DNA with rare-cutting restriction enzymes and in applying an alternating electrical current between spatially distinct pairs of electrodes. DNA molecules migrate at different speeds according to the size of the fragments. Among other things, this technique is considered as the “gold standard” for genotyping, genetic fingerprinting, epidemiological studies, genome size estimation, and studying radiation-induced DNA damage and repair. This chapter describes a PFGE method that can be used to differentiate dairy propionibacteria.
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Acknowledgments
The research leading to these results has received funding from the CNPq (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil) and CAPES (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil).
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Chuat, V., de Freitas, R., Dalmasso, M. (2015). Pulsed Field Gel Electrophoresis for Dairy Propionibacteria. In: Jordan, K., Dalmasso, M. (eds) Pulse Field Gel Electrophoresis. Methods in Molecular Biology, vol 1301. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2599-5_21
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DOI: https://doi.org/10.1007/978-1-4939-2599-5_21
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