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Pulse Field Gel Electrophoresis pp 241–251Cite as

Pulsed-Field Gel Electrophoresis for Leuconostoc mesenteroides and L. pseudomesenteroides

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1301))

Abstract

Pulsed-field gel electrophoresis (PFGE) is a technique using alternating electric fields to help the separation of high molecular weight DNA fragments with a high resolution. This method consists of the digestion of bacterial chromosomal DNA with rare-cutting restriction enzymes and of applying an alternating electrical current between spatially distinct pairs of electrodes. DNA molecules migrate at different speeds according to the size of the fragment. This method is considered as the “gold standard” for genotyping, genetic fingerprinting, epidemiological studies, genome size estimation, and studying radiation-induced DNA damage and repair.

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Acknowledgments

This research has received funding from the European Community’s Seventh Framework Programme (FP7, 2007–2013), Research Infrastructures action, under the grant agreement No. FP7-228310 (EMbaRC project). The authors would like to thank Dr. Tomislav Pogacic for providing some of the Leuconostoc strains used in this EMbaRC project work, which contributed greatly to the development of this chapter.

Author information

Authors and Affiliations

  1. UMR1253 Science et Technologie du Lait et de l’Oeuf, CIRM-BIA, INRA, 35042, Rennes, France

    Victoria Chuat

  2. AGROCAMPUS OUEST, UMR1253 Science et Technologie du Lait et de l’Oeuf, 35042, Rennes, France

    Victoria Chuat

  3. Department of Microbiology & Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland

    Marion Dalmasso

Authors
  1. Victoria Chuat
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  2. Marion Dalmasso
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Corresponding author

Correspondence to Victoria Chuat .

Editor information

Editors and Affiliations

  1. Teagasc Food Research Center, Moorepark, Fermoy, Co. Cork, Cork, Ireland

    Kieran Jordan

  2. Department of Microbiology & Alimentary Pharmabiotic Centre, University College Cork, Co. Cork, Cork, Ireland

    Marion Dalmasso

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Chuat, V., Dalmasso, M. (2015). Pulsed-Field Gel Electrophoresis for Leuconostoc mesenteroides and L. pseudomesenteroides . In: Jordan, K., Dalmasso, M. (eds) Pulse Field Gel Electrophoresis. Methods in Molecular Biology, vol 1301. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2599-5_19

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  • DOI: https://doi.org/10.1007/978-1-4939-2599-5_19

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2598-8

  • Online ISBN: 978-1-4939-2599-5

  • eBook Packages: Springer Protocols

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