Nuclear Reprogramming

Methods and Protocols

  • Nathalie Beaujean
  • Hélène Jammes
  • Alice Jouneau

Part of the Methods in Molecular Biology book series (MIMB, volume 1222)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Vincent Brochard, Zichuan Liu
    Pages 1-14
  3. Nathalie Daniel, Patrick Chesné
    Pages 15-23
  4. Joon-Hee Lee, Walid E. Maalouf
    Pages 25-36
  5. Mayuko Kurome, Barbara Kessler, Annegret Wuensch, Hiroshi Nagashima, Eckhard Wolf
    Pages 37-59
  6. Amélie Bonnet-Garnier, Anne-Clémence Veillard, Bertrand Bed’Hom, Hélène Hayes, Janice Britton-Davidian
    Pages 83-99
  7. Hiroshi Kimura, Kazuo Yamagata
    Pages 127-147
  8. Olga Østrup, Hanne S. Pedersen, Hanne M. Holm, Poul Hyttel
    Pages 161-174
  9. Nathalie Peynot, Véronique Duranthon, Daulat Raheem Khan
    Pages 181-196
  10. Isabelle Dufort, Claude Robert, Marc-André Sirard
    Pages 197-208
  11. Xin Li, Bao-long Xia, Wei Li, Qi Zhou
    Pages 247-254

About this book

Introduction

Nuclear Reprogramming: Methods and Protocols, Second Edition includes not only classic methods to perform nuclear transfer in different species but also several techniques to assess the early and late development of the reconstructed embryos, at the cellular, molecular, and epigenetic level. Over the past several years, many technical improvements have been made to improve somatic cell nuclear transfer (SCNT) efficiency, all of which are reflected in the detailed chapters of this fully revised collection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and up-to-date, Nuclear Reprogramming: Methods and Protocols, Second Edition will be of interest not only to cloners but also to researchers concerned with studying the development of embryos.

Keywords

Epigenetics Nuclear transfer Reconstructed embryos SCNT cloning Somatic cell genome Somatic cell nuclear transfer (SCNT) Totipotency

Editors and affiliations

  • Nathalie Beaujean
    • 1
  • Hélène Jammes
    • 2
  • Alice Jouneau
    • 3
  1. 1.INRAJouy-en-JosasFrance
  2. 2.INRAJouy-en-JosasFrance
  3. 3.INRAJouy-en-JosasFrance

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-1594-1
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1593-4
  • Online ISBN 978-1-4939-1594-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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