Abstract
Somatic cell nuclear transfer (SCNT) using genetically modified donor cells facilitates the generation of tailored pig models for biomedical research and for xenotransplantation. Up to now, SCNT is the main way to generate gene-targeted pigs, since germ line-competent pluripotent stem cells are not available for this species. In this chapter, we introduce our routine workflow for the production of genetically engineered pigs, especially focused on the genetic modification of somatic donor cells, SCNT using in vitro matured oocytes, and laparoscopic embryo transfer.
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References
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Acknowledgement
Our projects involving the generation of genetically engineered pigs are/were financially supported by the German Research Council (FOR 535 “Xenotransplantation,” FOR 793 “Mechanisms of Fracture Healing in Osteoporosis,” Transregio-CRC 127 “Biology of xenogeneic cell, tissue and organ transplantation—from bench to bedside”), by the Federal Ministry for Education and Research (Leading-Edge Cluster “m4—Personalised Medicine and Targeted Therapies”), the Bavarian Research Council (FORZebRA, Az. 802-08), and by the Mukoviszidose Institut gemeinnützige Gesellschaft für Forschung und Therapieentwicklung mbH. Authors are members of COST Action BM1308 “Sharing Advances on Large Animal Models – SALAAM”.
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Kurome, M., Kessler, B., Wuensch, A., Nagashima, H., Wolf, E. (2015). Nuclear Transfer and Transgenesis in the Pig. In: Beaujean, N., Jammes, H., Jouneau, A. (eds) Nuclear Reprogramming. Methods in Molecular Biology, vol 1222. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1594-1_4
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DOI: https://doi.org/10.1007/978-1-4939-1594-1_4
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