Abstract
Epigenetic modifications such as DNA methylation and posttranslational histone modifications change drastically during embryonic development. To visualize histone modifications in living embryos, a Fab-based live endogenous modification labeling (FabLEM) technique has been developed. Here we describe the methods required for FabLEM experiments, including Fab preparation from IgG, its conjugation with a fluorescent dye, loading into cultured cells or mouse embryos, and imaging.
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Acknowledgement
We thank Yuko Sato, Timothy J. Stasevich, and Yoko Hayashi-Takanaka for comments, figures, and data. The authors’ work was supported by grants-in-aid from the Ministry of Education, Culture, Sports, Science and Technology, Japan Science and Technology Agency Core Research for Evolutional Science and Technology, and New Energy and Industrial Technology Development Organization.
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Kimura, H., Yamagata, K. (2015). Visualization of Epigenetic Modifications in Preimplantation Embryos. In: Beaujean, N., Jammes, H., Jouneau, A. (eds) Nuclear Reprogramming. Methods in Molecular Biology, vol 1222. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1594-1_10
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DOI: https://doi.org/10.1007/978-1-4939-1594-1_10
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