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Nuclear Reprogramming pp 101–111Cite as

Treatment of Donor Cell/Embryo with Different Approaches to Improve Development After Nuclear Transfer

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1222))

Abstract

The successful production of cloned animals by somatic cell nuclear transfer (SCNT) is a promising technology with many potential applications in basic research, medicine, and agriculture. However, the low efficiency and the difficulty of cloning are major obstacles to the widespread use of this technology. Since the first mammal cloned from an adult donor cell was born, many attempts have been made to improve animal cloning techniques, and some approaches have successfully improved its efficiency. Nuclear transfer itself is still difficult because it requires an accomplished operator with a practiced technique. Thus, it is very important to find simple and reproducible methods for improving the success rate of SCNT. In this chapter, we will review our recent protocols, which seem to be the simplest and most reliable method to date to improve development of SCNT embryos.

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Author information

Authors and Affiliations

  1. University of Yamanashi, Kofu Campus, 4-4-37 Takeda, Kofu, Yamanashi, 400-8510, Japan

    Eiji Mizutani, Sayaka Wakayama & Teruhiko Wakayama

Authors
  1. Eiji Mizutani
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  2. Sayaka Wakayama
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  3. Teruhiko Wakayama
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Corresponding author

Correspondence to Eiji Mizutani .

Editor information

Editors and Affiliations

  1. INRA, Jouy-en-Josas, France

    Nathalie Beaujean

  2. INRA, Jouy-en-Josas, France

    Hélène Jammes

  3. INRA, Jouy-en-Josas, France

    Alice Jouneau

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Mizutani, E., Wakayama, S., Wakayama, T. (2015). Treatment of Donor Cell/Embryo with Different Approaches to Improve Development After Nuclear Transfer. In: Beaujean, N., Jammes, H., Jouneau, A. (eds) Nuclear Reprogramming. Methods in Molecular Biology, vol 1222. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1594-1_8

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  • DOI: https://doi.org/10.1007/978-1-4939-1594-1_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1593-4

  • Online ISBN: 978-1-4939-1594-1

  • eBook Packages: Springer Protocols

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