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Flow Cytometry Protocols

  • Book
  • © 1998

Overview

Editors:
  1. Mark J. Jaroszeski

    1. University of South Florida, Tampa

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Richard Heller

    1. University of South Florida, Tampa

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 91)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Basics of Flow Cytometry

    • Gilbert Radcliff, Mark J. Jaroszeski
    Pages 1-24

  3. Detection of Terminal Transferase in Leukemia

    • Elisabeth Paietta
    Pages 25-36

  4. The Use of Flow Cytometry to Detect Intracellular Cytokine Production in Individual Cells

    • Brian E. Crucian, Raymond H. Widen
    Pages 37-46

  5. Detection of Intracellular/lntranuclear Antigens

    • Raymond H. Widen
    Pages 47-55

  6. Detection of Intracellular/lntranuclear Antigens in Tumor Cells

    • Raymond H. Widen, Jeanne L. Becker
    Pages 57-65

  7. Detection of Cyclins in Individual Cells by Flow and Laser Scanning Cytometry

    • Gloria Juan, Zbigniew Darzynkiewicz
    Pages 67-75

  8. Estimation of Cell Viability by Flow Cytometry

    • James L. Weaver
    Pages 77-83

  9. Flow Cytometric Detection of Fluorescent Redistributional Dyes for Measurement of Cell Transmembrane Potential

    • Michael K. Tanner, Samuel R. Wellhausen
    Pages 85-95

  10. Flow Cytometric Analysis of Macrophage Oxidative Metabolism Using DCFH

    • Amy Imrich, Lester Kobzik
    Pages 97-108

  11. Measurement of Environmental Particulate Uptake by Lung Cells Using Flow Cytometry

    • Bradley Stringer, Lester Kobzik
    Pages 109-116

  12. Estimation of Drug Resistance by Flow Cytometry

    • Adorjan Aszalos, James L. Weaver
    Pages 117-122

  13. Flow Cytometric Monitoring of Cellular Pharmacokinetics

    • Margaret S. Dordal
    Pages 123-132

  14. Quantification of Xenoprotein Electroinsertion in Mammalian Cells

    • mnKhalid El Ouagari, Justin Teissié
    Pages 133-139

  15. Flow Cytometry Quantification of Electropermeabilization

    • Marie-Pierre Rols, Justin Teissié
    Pages 141-147

  16. Flow Cytometric Detection and Quantitation of Cell-Cell Electrofusion Products

    • Mark J. Jaroszeski, Richard Gilbert, Richard Heller
    Pages 149-156

  17. Cell Cycle Analysis of Asynchronous Populations

    • Michael G. Ormerod
    Pages 157-165

  18. Simultaneous DNA/RNA Analysis of Solid and Hematoreticular Malignancies

    • Kim Steck, Adel El-Naggar
    Pages 167-179

  19. Solid Tumor DNA Ploidy Analysis

    • Kim Steck, Adel El-Naggar
    Pages 181-195

  20. FISHing for Cytokines

    • Kenneth J. Pennline
    Pages 197-215
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About this book

Flow cytometers are technically sophisticated instruments that utilize pr- ciples of physics, chemistry, engineering, digital electronics, optics, analog devices, laser technology, and biology to measure the light scattering and fluor- cent characteristics of particles. These instruments have become invaluable tools in the area of cancer research, cell biology, and clinical diagnostics for analyzing a multitude of cellular and subcellular particles. Undoubtedly, flow cytometers have become widely used because they provide a very unique means of exam- ing biological particles. Large quantities of cells/particles are individually a- lyzed, and it is typical for flow cytometers to quantitatively process thousands of individual particles in a matter of seconds. This a powerful analytic feat parti- larly if one relates it to the time required to examine several thousand individual cells using a microscope. This leaves little doubt regarding why the field of flow cytometry has advanced so rapidly since the first flow cytometers were commerically available in the 1970s. Today, flow cytometers are common in both large and small institutions. Current flow cytometers have evolved with advances in computers and microelectronics into fast and reliable instruments. The science of flow cytometry has also benefitted from rapid growth in the fields of biochemistry and immunology over the past decade. A vast array of fluorochrome-con- gated antibodies and other reagents are now commercially available for pr- ing structures and monitoring function in human cells as as in many other species.

Reviews

"...editors M.J. Jaroszeski and R. Heller succed in assembling a representative collection of protocols focused on structural and functional analysis of human and animal cells...is well arranged and produced."-Reviewed by J. Dolezel, Biologia Plantarum

Editors and Affiliations

  • University of South Florida, Tampa

    Mark J. Jaroszeski, Richard Heller

Bibliographic Information

  • Book Title: Flow Cytometry Protocols

  • Editors: Mark J. Jaroszeski, Richard Heller

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896033546

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1998

  • eBook ISBN: 978-1-59259-214-2Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 274

  • Number of Illustrations: 56 b/w illustrations

  • Topics: Cell Biology

  • Industry Sectors: Biotechnology, Pharma

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