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Detection of Cyclins in Individual Cells by Flow and Laser Scanning Cytometry

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Flow Cytometry Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 91))

Abstract

Progression of cells through successive phases and checkpoints of the cell cycle is maintained by sequential phosphorylation of different sets of nuclear and cytoplasmic proteins by cyclin-dependent kinases (CDKs) (1–12). By activating their partner CDKs and targeting them to the respective protein substrates cyclins play a key regulatory role in this process. Cyclins Bl, A, E, and D are expressed discontinuously during the cycle. The synthesis and degradation of these cyclins occurs at well-defined time points of the cell cycle (Table 1).

Table 1 Cyclins and Their Partner CDKs During the Cell Cycle
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Author information

Authors and Affiliations

  1. The Cancer Research Institute, New York Medical College, Elmsford, NY

    Gloria Juan & Zbigniew Darzynkiewicz

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  1. Gloria Juan
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  2. Zbigniew Darzynkiewicz
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Editor information

Editors and Affiliations

  1. University of South Florida, Tampa, FL

    Mark J. Jaroszeski  & Richard Heller  & 

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© 1998 Humana Press Inc., Totowa, NJ

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Juan, G., Darzynkiewicz, Z. (1998). Detection of Cyclins in Individual Cells by Flow and Laser Scanning Cytometry. In: Jaroszeski, M.J., Heller, R. (eds) Flow Cytometry Protocols. Methods in Molecular Biology™, vol 91. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-354-6:67

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  • DOI: https://doi.org/10.1385/0-89603-354-6:67

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-354-2

  • Online ISBN: 978-1-59259-214-2

  • eBook Packages: Springer Protocols

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