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Detection of Intracellular/lntranuclear Antigens

Applications in Leukemia/Lymphoma Analysis

  • Protocol
Flow Cytometry Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 91))

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Abstract

The increased utilization of flow cytometry for the study of cell-associated antigens has paralleled the continuing development of new monoclonal antibodies (MAbs) specific for cell-associated antigens. Availability of these reagents, combined with the introduction of newer fluorochromes such as phycoerythrin (PE) and peridmin chlorophyll (PERCp) or tandem conjugates such as PE-Cy5 that may be used in conjunction with fluorescein isothiocyanate (FITC) allow simultaneous measurement of coexpression of three or four different antigens. Flow cytometry instrumentation also has evolved to the point that three- and four-color analysis is routinely available in simple benchtop instruments, not just on high powered sorting instruments.

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Author information

Authors and Affiliations

  1. Immunology Department, Tampa General Hospital, Tampa, FL

    Raymond H. Widen

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  1. Raymond H. Widen
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Editor information

Editors and Affiliations

  1. University of South Florida, Tampa, FL

    Mark J. Jaroszeski  & Richard Heller  & 

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© 1998 Humana Press Inc., Totowa, NJ

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Widen, R.H. (1998). Detection of Intracellular/lntranuclear Antigens. In: Jaroszeski, M.J., Heller, R. (eds) Flow Cytometry Protocols. Methods in Molecular Biology™, vol 91. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-354-6:47

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  • DOI: https://doi.org/10.1385/0-89603-354-6:47

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-354-2

  • Online ISBN: 978-1-59259-214-2

  • eBook Packages: Springer Protocols

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