Abstract
Cell-cell electrofusion (CCE) is a process that involves forcing cells into close juxtaposition and then inducing fusion by delivering electric pulses to the cells. CCE has proven to have many practical applications. It has been used for monoclonal antibody (Mab) production (4,5), hybridoma production (1–3), and membrane surface marker transfer (6). Many other applications are described in this volume. In addition, the study of membrane fusion mechanisms has been the focus of some researchers (7–12). Electrofusion techniques seldom result in 100% yields between fusion partners. Therefore, a major aspect of all CCE applications is the ability to detect and quantitate fusion products.
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© 1998 Humana Press Inc., Totowa, NJ
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Jaroszeski, M.J., Gilbert, R., Heller, R. (1998). Flow Cytometric Detection and Quantitation of Cell-Cell Electrofusion Products. In: Jaroszeski, M.J., Heller, R. (eds) Flow Cytometry Protocols. Methods in Molecular Biology™, vol 91. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-354-6:149
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DOI: https://doi.org/10.1385/0-89603-354-6:149
Publisher Name: Humana Press, Totowa, NJ
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