Overview
- Editors:
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Tom Moss
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Centre de Recherche en Cancérologie de l’Université Laval, Centre Hopital Universitaire de Québec et Départment de biologie médicale, Université Laval, Québec, Canada
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Table of contents (43 protocols)
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Front Matter
Pages i-xvii
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- Marc-André Laniel, Alain Béliveau, Sylvain L. Guérin
Pages 13-30
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- Willi Metzger, Hermann Heumann
Pages 39-47
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- Evgeny Zaychikov, Peter Schickor, Ludmilla Denissova, Hermann Heumann
Pages 49-61
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- Brenda F. Kahl, Marvin R. Paule
Pages 63-75
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- Athanasios G. Papavassiliou
Pages 77-110
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- Athanasios G. Papavassiliou
Pages 135-149
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- Malcolm Buckle, Andrew A. Travers
Pages 151-160
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- Johannes Geiselmann, Frederic Boccard
Pages 161-173
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- Régen Drouin, Jean-Philippe Therrien, Martin Angers, Stéphane Ouellet
Pages 175-219
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- Peter E. Shaw, A. Francis Stewart
Pages 221-227
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- Ian W. Manfield, Peter G. Stockley
Pages 229-243
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- Peter Schickor, Evgeny Zaychikov, Hermann Heumann
Pages 245-253
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- Simon Labbé, Gale Stewart, Olivier LaRochelle, Carl Séguin, Guy G. Poirier
Pages 255-264
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- Ian A. Taylor, G. Geoff Kneale
Pages 265-274
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- David R. Chafin, Jeffrey J. Hayes
Pages 275-290
About this book
Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions and define their components, their manner of interaction, and their manner of function, both in the cell and in the test tube. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to understand DNA-protein interactions.
Reviews
"The book, written by an impressive list of worldwide contributors, will provide readers with nearly all the necessary tools for studying DNA-protein interactions, making it a perfect cell laboratory manual." -Quarterly Review of Biology
"...a great laboratory resource for biochemists and molecular, cellular, and developmental biologists." - Today's Life Science
Editors and Affiliations
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Centre de Recherche en Cancérologie de l’Université Laval, Centre Hopital Universitaire de Québec et Départment de biologie médicale, Université Laval, Québec, Canada
Tom Moss