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  • Book
  • © 2001

DNA'Protein Interactions

Principles and Protocols

Editors:

  1. Tom Moss

    1. Centre de Recherche en Cancérologie de l’Université Laval, Centre Hopital Universitaire de Québec et Départment de biologie médicale, Université Laval, Québec, Canada

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Part of the book series: Methods in Molecular Biology (MIMB, volume 148)

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Table of contents (43 protocols)

  2. Chemical Modification of Lysine by Reductive Methylation

    • Ian A. Taylor, Michelle Webb
    Pages 301-314

  3. Limited Proteolysis of Protein-Nucleic Acid Complexes

    • Simon E. Plyte, G. Geoff Kneale
    Pages 315-321

  4. Ultraviolet Crosslinking of DNA-Protein Complexes via 8-Azidoadenine

    • Rainer Meffert, Klaus Dose, Gabriele Rathgeber, Hans-Jochen Schäfer
    Pages 323-335

  5. Site-Specific Protein-DNA Photocrosslinking

    • Nikolai Naryshkin, Younggyu Kim, Qianping Dong, Richard H. Ebright
    Pages 337-361

  8. UV Laser-Induced Protein-DNA Crosslinking

    • Stefan I. Dimitrov, Tom Moss
    Pages 395-402

  9. Plasmid Vectors for the Analysis of Protein-Induced DNA Bending

    • Christian Zwieb, Sankar Adhya
    Pages 403-416

  11. Genetic Analysis of DNA-Protein Interactions Using a Reporter Gene Assay in Yeast

    • David R. Setzer, Deborah B. Schulman, Michael J. Bumbulis
    Pages 431-449

  12. Assays for Transcription Factor Activity

    • Virgil Rhodius, Nigel Savery, Annie Kolb, Stephen Busby
    Pages 451-464

  13. Assay of Restriction Endonucleases Using Oligonucleotides

    • Bernard A. Connolly, Hsiao-Hui Liu, Damian Parry, Lisa E. Engler, Michael R. Kurpiewski, Linda Jen-Jacobson
    Pages 465-490

  14. Analysis of DNA-Protein Interactions by Intrinsic Fluorescence

    • Mark L. Carpenter, Anthony W. Oliver, G. Geoff Kneale
    Pages 491-502

  15. Circular Dichroism for the Analysis of Protein-DNA Interactions

    • Mark L. Carpenter, Anthony W. Oliver, G. Geoff Kneale
    Pages 503-510

  16. Calorimetry of Protein-DNA Complexes and Their Components

    • Christopher M. Read, Ilian Jelesarov
    Pages 511-533

  18. Reconstitution of Protein-DNA Complexes for Crystallization

    • Rachel M. Conlin, Raymond S. Brown
    Pages 547-556

  19. Two-Dimensional Crystallization of Soluble Protein Complexes

    • Patrick Schultz, Nicolas Bischler, Luc Lebeau
    Pages 557-568

  20. Atomic Force Microscopy of DNA and Protein-DNA Complexes Using Functionalized Mica Substrates

    • Yuri L. Lyubchenko, Alexander A. Gall, Luda S. Shlyakhtenko
    Pages 569-578
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About this book

Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions and define their components, their manner of interaction, and their manner of function, both in the cell and in the test tube. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to understand DNA-protein interactions.
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Keywords

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Reviews

"The book, written by an impressive list of worldwide contributors, will provide readers with nearly all the necessary tools for studying DNA-protein interactions, making it a perfect cell laboratory manual." -Quarterly Review of Biology

"...a great laboratory resource for biochemists and molecular, cellular, and developmental biologists." - Today's Life Science
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Editors and Affiliations

  • Centre de Recherche en Cancérologie de l’Université Laval, Centre Hopital Universitaire de Québec et Départment de biologie médicale, Université Laval, Québec, Canada

    Tom Moss

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Bibliographic Information

  • Book Title: DNA'Protein Interactions

  • Book Subtitle: Principles and Protocols

  • Editors: Tom Moss

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592592082

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2001

  • eBook ISBN: 978-1-59259-208-1Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: 656

  • Number of Illustrations: 146 b/w illustrations

  • Topics: Cell Biology

  • Industry Sectors: Biotechnology, Pharma

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Buy it now

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

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