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Uranyl Photofootprinting

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DNA-Protein Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 148))

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Abstract

It has long been known that the uranyl(VI) ion (UO2 2+) forms strong complexes with various inorganic and organic anions, including phosphates, and that the photochemically excited state of this ion is a very strong oxidant (1). For instance, uranyl-mediated photooxidation of alcohols has been studied in detail (2,3). It is also widely recognized that uranyl chemistry and photophysics/photochemistry are very complex. Thus monomeric UO2 2+is only present at low pH (pH approx 2), whereas polynuclear species and various “hydroxides,” which often precipitate, form at a higher pH (4).

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Author information

Authors and Affiliations

  1. Department of Medical Biochemistry and Genetics, Laboratory of Biochemistry, The Panum Institute, Copenhagen, Denmark

    Peter E. Nielsen

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  1. Peter E. Nielsen
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Editors and Affiliations

  1. Centre de Recherche en Cancérologie de l’Université Laval, Centre Hopital Universitaire de Québec et Départment de biologie médicale, Université Laval, Québec, QC, Canada

    Tom Moss

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© 2001 Humana Press Inc., Totowa, NJ

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Nielsen, P.E. (2001). Uranyl Photofootprinting. In: Moss, T. (eds) DNA-Protein Interactions. Methods in Molecular Biology, vol 148. Humana Press. https://doi.org/10.1385/1-59259-208-2:111

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  • DOI: https://doi.org/10.1385/1-59259-208-2:111

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-625-3

  • Online ISBN: 978-1-59259-208-1

  • eBook Packages: Springer Protocols

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