DNA-Protein Interactions

Principles and Protocols

  • Tom Moss

Part of the Methods in Molecular Biology book series (MIMB, volume 148)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Peter G. Stockley
    Pages 1-11
  3. Marc-André Laniel, Alain Béliveau, Sylvain L. Guérin
    Pages 13-30
  4. Benoît Leblanc, Tom Moss
    Pages 31-38
  5. Willi Metzger, Hermann Heumann
    Pages 39-47
  6. Evgeny Zaychikov, Peter Schickor, Ludmilla Denissova, Hermann Heumann
    Pages 49-61
  7. Peter E. Nielsen
    Pages 111-119
  8. Malcolm Buckle, Andrew A. Travers
    Pages 151-160
  9. Johannes Geiselmann, Frederic Boccard
    Pages 161-173
  10. Régen Drouin, Jean-Philippe Therrien, Martin Angers, Stéphane Ouellet
    Pages 175-219
  11. Peter E. Shaw, A. Francis Stewart
    Pages 221-227
  12. Ian W. Manfield, Peter G. Stockley
    Pages 229-243
  13. Peter Schickor, Evgeny Zaychikov, Hermann Heumann
    Pages 245-253
  14. Simon Labbé, Gale Stewart, Olivier LaRochelle, Carl Séguin, Guy G. Poirier
    Pages 255-264
  15. Ian A. Taylor, G. Geoff Kneale
    Pages 265-274
  16. David R. Chafin, Jeffrey J. Hayes
    Pages 275-290

About this book

Introduction

DNA-protein interactions are fundamental to understanding biological growth, development, differentiation, evolution, and disease, and are also increasingly important to the biotechnology industry. In DNA-Protein Interactions: Principles and Protocols, 2nd Edition, Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions, define their components, and determine the nature of their interactions and their manner of function, both in the cell and in the test tube. Each detailed method has been optimized and tested by a hands-on expert to ensure experimental success, and provides helpful notes on pitfalls to avoid, as well as alternate strategies. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. There are also vanguard methods for designing proteins to fit DNA, site-specific protein-directed DNA cleavage, and 2D semicrystalline arrays of nucleoproteins for structure determination.
Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to illuminate DNA-protein interactions.

Keywords

DNA RNA Termination Vivo Western Blotting bacteria cell development gene genes nucleic acid protein reproducible techniques transcription

Editors and affiliations

  • Tom Moss
    • 1
  1. 1.Centre de Recherche en Cancérologie de l’Université LavalCentre Hopital Universitaire de Québec et Départment de biologie médicale, Université LavalQuébecCanada

Bibliographic information

  • DOI https://doi.org/10.1385/1592592082
  • Copyright Information Humana Press 2001
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-625-3
  • Online ISBN 978-1-59259-208-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
Industry Sectors
Biotechnology
Consumer Packaged Goods
Pharma