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Molecular Toxicology Protocols

  • Book
  • © 2005

Overview

Editors:
  1. Phouthone Keohavong

    1. Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh

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    You can also search for this editor in PubMed   Google Scholar

  2. Stephen G. Grant

    1. Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 291)

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Table of contents (38 protocols)

  1. Detection and Characterization of Surrogate Gene Mutation

    1. Simultaneous Quantification of t(14;18) and HPRT Exon 2/3 Deletions in Human Lymphocytes

      • James C. Fuscoe
      Pages 171-178

    2. The GPA In Vivo Somatic Mutation Assay

      • Stephen G. Grant
      Pages 179-195

    3. Flow Cytometric Measurement of Mutant T Cells With Altered Expression of TCR

      • Seishi Kyoizumi, Yoichiro Kusunoki, Tomonori Hayashi
      Pages 197-204

  2. Detection and Characterization of Cancer Gene Mutation

    1. Front Matter

      Pages 205-205
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    2. Mutation Screening of the TP53 Gene by Temporal Temperature Gradient Gel Electrophoresis

      • Therese Sørlie, Hilde Johnsen, Phuong Vu, Guro Elisabeth Lind, Ragnhild Lothe, Anne-Lise Børresen-Dale
      Pages 207-216

    3. Analysis of K-RAS and P53 Mutations in Sputum Samples

      • Weimin Gao, Phouthone Keohavong
      Pages 217-233

    4. Allele-Specific Competitive Blocker-PCR Detection of Rare Base Substitution

      • Barbara L. Parsons, Page B. McKinzie, Robert H. Heflich
      Pages 235-245

    5. Gel-Based Nonradioactive Single-Strand Conformational Polymorphism and Mutation Detection

      • Vibhuti Gupta, Reetakshi Arora, Anand Ranjan, Narendra K. Bairwa, Dheeraj K. Malhotra, P. T. Udhayasuriyan et al.
      Pages 247-261

    6. Detection and Characterization of Oncogene Mutations in Preneoplastic and Early Neoplastic Lesions

      • Toshinari Minamoto
      Pages 263-278

    7. Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR

      • Michael J. Villalobos, Christopher J. Betti, Andrew T. M. Vaughan
      Pages 279-289

  3. Analysis of DNA Repair Mechanisms

    1. Front Matter

      Pages 291-291
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    2. Microsatellite Instability

      • Anjana Saha, Narendra K. Bairwa, Ramesh Bamezai
      Pages 293-302

    3. Unscheduled DNA Synthesis

      • Crystal M. Kelly, Jean J. Latimer
      Pages 303-320

    4. Analysis of DNA Repair Using Transfection-Based Host Cell Reactivation

      • Jennifer M. Johnson, Jean J. Latimer
      Pages 321-335

    5. An Immunoassay for Measuring Repair of Ultraviolet Photoproducts

      • Shirley McCready
      Pages 337-349

    6. Analysis of DNA Double-Strand Break Repair by Nonhomologous End Joining in Cell-Free Extracts From Mammalian Cells

      • Petra Pfeiffer, Elke Feldmann, Andrea Odersky, Steffi Kuhfittig-Kulle, Wolfgang Goedecke
      Pages 351-371

    7. Measuring Recombination Proficiency in Mouse Embryonic Stem Cells

      • Andrew J. Pierce, Maria Jasin
      Pages 373-384

  4. Array Technologies

    1. Front Matter

      Pages 385-385
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    2. Strategies for Measurement of Biotransformation Enzyme Gene Expression

      • Marjorie Romkes, Shama C. Buch
      Pages 387-398

    3. Genotyping Technologies

      • Marjorie Romkes, Shama C. Buch
      Pages 399-414
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Keywords

About this book

A collection of cutting-edge techniques for analyzing genotoxic exposure and detecting the resulting biological effects-including endogenous metabolites-up to and including the development of cancer. The authors emphasize analytical methods that can be specifically applied to human populations and patients. Among the applications detailed are the analysis of interactions between such cellular macromolecules as DNA and proteins and chemical and physical agents, the assessment of medically relevant toxicity, and the characterization of genetic alterations induced in transgenic animals by in vivo systems. There are also methods for the analysis of genotoxic exposure during gene expression, of cytotoxicity caused by the induction of apoptosis, of genetic alterations in reporter genes and oncogenes, early (premalignant) detection of altered oncogenes, and of individual variation in biotransformation and DNA repair capacity.

Reviews

"This book should be of interest to both specialist researchers as well as members of the wider scientific community..." - British Journal of Anesthesiology

Editors and Affiliations

  • Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh

    Phouthone Keohavong, Stephen G. Grant

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