Abstract
A method is presented to measure homologous recombination in mouse embryonic stem cells by both gene targeting and short-tract gene conversion of a double-strand break. A fluorescence-based reporter is first gene targeted to the Hprt locus in a quantifiable way. A homing endonuclease expression vector is then introduced to generate a double-strand break, the repair of which is also quantifiable.
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© 2005 Humana Press Inc.
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Pierce, A.J., Jasin, M. (2005). Measuring Recombination Proficiency in Mouse Embryonic Stem Cells. In: Keohavong, P., Grant, S.G. (eds) Molecular Toxicology Protocols. Methods in Molecular Biology™, vol 291. Humana Press. https://doi.org/10.1385/1-59259-840-4:373
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DOI: https://doi.org/10.1385/1-59259-840-4:373
Publisher Name: Humana Press
Print ISBN: 978-1-58829-084-7
Online ISBN: 978-1-59259-840-3
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