Overview
- Editors:
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Jonathan M. Gibbins
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School of Animal and Microbial Sciences, The University of Reading, Reading, UK
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Martyn P. Mahaut-Smith
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Department of Physiology, University of Cambridge, Cambridge, UK
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About this book
12 The average human body has on the order of 10 circulating platelets. They are crucial for hemostasis, and yet excessive platelet activation is a major cause of m- bidity and mortality in Western societies. It is therefore not surprising that platelets have become one of the most extensively investigated biological cell types. We are, however, far from understanding precisely how platelets become activated under physiological and pathophysiological conditions. In addition, there are large gaps in our knowledge of platelet production from their giant precursor cell, the megakar- cyte. Understanding megakaryocyte biology will be crucial for the development of platelet gene targeting. The aim of Platelets and Megakaryocytes is therefore to bring together established and recently developed techniques to provide a comprehensive guide to the study of both the platelet and the megakaryocyte. It consists of five s- tions split between two volumes. The more functional assays appear in Volume 1, whereas Volume 2 includes signaling techniques, postgenomic methods, and a n- ber of key perspectives chapters. Part I of Volume 1, Platelets and Megakaryocytes: Functional Assays, describes many well-established approaches to the study of platelet function, including aggregometry, secretion, arachidonic acid metabolism, procoagulant responses, pla- let adhesion under static or flow conditions, flow cytometry, and production of microparticles. Although one would ideally wish to perform experiments with human platelets, studies within the circulation using intravital microscopy require the use of animal models, which are described in Chapter 16, vol. 1.
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Table of contents (29 protocols)
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Perspectives
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- Donna Woulfe, Jing Yang, Nicolas Prevost, Peter O’Brien, Ryan Fortna, Massimiliano Tognolini et al.
Pages 3-31
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- Nikla R. Emambokus, George J. Murphy, Jonathan Frampton
Pages 33-55
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- José Rivera, MarÃa L. Lozano, Vicente Vicente
Pages 57-72
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- Chris J. Vlahos, Louis F. Stancato
Pages 87-102
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Signaling Techniques
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Front Matter
Pages 103-103
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- Stephanie M. Jung, Masaaki Moroi
Pages 105-114
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- Pierre Savi, Jean-Marc Herbert
Pages 115-124
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- Matthew L. Jones, Alastair W. Poole
Pages 169-177
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- Gertie Gorter, Jan Willem Akkerman
Pages 179-199
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- Corie N. Shrimpton, Karine Gousset, Fern Tablin, José A. López
Pages 213-228
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- Philippe Ohlmann, Béatrice Hechler, Jean-Pierre Cazenave, Christian Gachet
Pages 229-250
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- Michael J. Mason, Martyn P. Mahaut-Smith
Pages 251-275
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Reviews
"...thorough and detailed...contains useful information regarding the study of platelets and megakaryocytes." - Veterinary Pathology
Editors and Affiliations
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School of Animal and Microbial Sciences, The University of Reading, Reading, UK
Jonathan M. Gibbins
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Department of Physiology, University of Cambridge, Cambridge, UK
Martyn P. Mahaut-Smith