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Measurement and Manipulation of [Ca2+]i in Suspensions of Platelets and Cell Cultures

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Platelets and Megakaryocytes

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 273))

Abstract

The importance of cytosolic calcium (Ca2+) elevation in the regulation of platelet functions (shape change, aggregation, and secretion) has been widely acknowledged. Therefore its concentration must be tightly regulated, with most of it being sequestrated in intracellular organelles such as the dense tubular system (the releasable intracellular Ca2+ store) and mitochondria, or bound to membranes and cytoplasmic proteins. Only a small fraction is freely available in the ionized form. The maintenance of low cytosolic calcium concentration (around 100 nM), necessary to keep platelets in a resting state or to reestablish a resting state after activation, is achieved by a combination of Ca2+/ATPases and a Na+/Ca2+ exchanger. Platelets contain two types of Ca2+/ATPases: the plasma membrane Ca2+/ATPase (PMCA types 1–4) (1) and the sarco-endoplasmic reticulum Ca2+/ATPase (SERCA) present on intracellular stores (2). Two types of Na+/Ca2+ exchangers have been identified; the platelets have been suggested to contain the retinal type, which is K+-dependent and located in the plasma membrane (3,4).

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Authors and Affiliations

  1. INSERM U.311, Etablissement Français du Sang-Alsace, Strasbourg, France

    Philippe Ohlmann, Béatrice Hechler, Jean-Pierre Cazenave & Christian Gachet

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  1. Philippe Ohlmann
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  2. Béatrice Hechler
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  3. Jean-Pierre Cazenave
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  4. Christian Gachet
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Editors and Affiliations

  1. School of Animal and Microbial Sciences, The University of Reading, Reading, UK

    Jonathan M. Gibbins

  2. Department of Physiology, University of Cambridge, Cambridge, UK

    Martyn P. Mahaut-Smith

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Ohlmann, P., Hechler, B., Cazenave, JP., Gachet, C. (2004). Measurement and Manipulation of [Ca2+]i in Suspensions of Platelets and Cell Cultures. In: Gibbins, J.M., Mahaut-Smith, M.P. (eds) Platelets and Megakaryocytes. Methods in Molecular Biology™, vol 273. Humana Press. https://doi.org/10.1385/1-59259-783-1:229

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  • DOI: https://doi.org/10.1385/1-59259-783-1:229

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-011-3

  • Online ISBN: 978-1-59259-783-3

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