Receptor Signal Transduction Protocols

  • Gary B. Willars
  • R. A. John Challiss

Part of the Methods in Molecular Biology book series (MIMB, volume 259)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. David B. Bylund, Jean D. Deupree, Myron L. Toews
    Pages 1-28
  3. R. A. Jeffrey McIlhinney
    Pages 81-98
  4. Antonio Porcellini, Antonio De Blasi
    Pages 155-166
  5. Martin Hohenegger, Michael Freissmuth, Christian Nanoff
    Pages 183-195
  6. Ian Mullaney, Graeme Milligan
    Pages 207-224
  7. Jennifer A. Koenig
    Pages 249-273
  8. Andrew B. Tobin, Mark Wheatley
    Pages 275-281
  9. Adriano Marchese, Jeffrey L. Benovic
    Pages 299-305
  10. Marion Blomenröhr, Henry F. Vischer, Jan Bogerd
    Pages 307-322

About this book

Introduction

In this second edition of a widely appreciated work, Receptor Signal Transduction Protocols, a panel of internationally recognized investigators presents their best methods for studying G-protein-coupled receptors (GPCRs) and events immediately downstream of their activation. This new edition combines updates of key chapters from the first edition with a large number of new contributions on the many successful methodologies that have emerged more recently. The methods are focused primarily on events at the receptor level, including ligand binding, on the genetic manipulation of receptors, the generation of model cell lines in which to study them, and the interaction and activation of G-proteins. Additional methods concentrate on receptor expression and localization, receptor internalization and post-translational modification, GPCR-protein interactions, and the use of knock-out and knock-in strategies for determining the physiological roles of receptors. The laboratory protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Receptor Signal Transduction Protocols, Second Edition offers both novice and experienced investigators powerful cutting-edge techniques that provide an array of approaches and specific methods that will aid in the understanding of GPCR structure and function.

Editors and affiliations

  • Gary B. Willars
    • 1
  • R. A. John Challiss
    • 1
  1. 1.Department of Cell Physiology and PharmacologyUniversity of LeicesterLeicesterUK

Bibliographic information

  • DOI https://doi.org/10.1385/1592597548
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-329-9
  • Online ISBN 978-1-59259-754-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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