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Generation of Model Cell Lines Expressing Recombinant G-Protein-Coupled Receptors

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Receptor Signal Transduction Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 259))

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Abstract

The molecular cloning of the cDNA sequences encoding most G-protein-coupled receptors, including those from humans, allows their study in a variety of recombinant systems. In this respect, transfected mammalian cell lines constitute the most frequently used model for investigating the pharmacological and biochemical properties of these receptors. Several protocols have been described (based on the use of calcium phosphate precipitation, DEAE dextran, cationic lipids, and electroporation), allowing their transient or stable expression in diverse cell lines. This chapter gives a brief overview of the different techniques and provides methodology for the generation of transiently transfected cells and for selection, isolation and maintenance of stable transfected cell lines.

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Author information

Authors and Affiliations

  1. Laboratory of Experimental Pharmacology, Catholic University of Louvain, Brussels, Belgium

    Emmanuel Hermans

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  1. Emmanuel Hermans
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Editor information

Editors and Affiliations

  1. Department of Cell Physiology and Pharmacology, University of Leicester, Leicester, UK

    Gary B. Willars  & R. A. John Challiss  & 

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© 2004 Humana Press Inc.

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Hermans, E. (2004). Generation of Model Cell Lines Expressing Recombinant G-Protein-Coupled Receptors. In: Willars, G.B., Challiss, R.A.J. (eds) Receptor Signal Transduction Protocols. Methods in Molecular Biology, vol 259. Humana Press. https://doi.org/10.1385/1-59259-754-8:137

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  • DOI: https://doi.org/10.1385/1-59259-754-8:137

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-329-9

  • Online ISBN: 978-1-59259-754-3

  • eBook Packages: Springer Protocols

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