Abstract
The radioligand-binding assay is a relatively simple but powerful tool for studying G-protein-coupled receptors. There are three basic types of radioligand-binding experiments: (1) saturation experiments from which the affinity of the radioligand for the receptor and the binding site density can be determined; (2) inhibition experiments from which the affinity of a competing, unlabeled compound for the receptor can be determined; and (3) kinetic experiments from which the forward and reverse rate constants for radioligand binding can be determined. Detailed methods for typical radioligand-binding assays for G-protein-coupled receptors in membranes and intact cells are presented for these types of experiments. Detailed procedures for analysis of the data obtained from these experiments are also given.
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© 2004 Humana Press Inc.
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Bylund, D.B., Deupree, J.D., Toews, M.L. (2004). Radioligand-Binding Methods for Membrane Preparations and Intact Cells. In: Willars, G.B., Challiss, R.A.J. (eds) Receptor Signal Transduction Protocols. Methods in Molecular Biology, vol 259. Humana Press. https://doi.org/10.1385/1-59259-754-8:001
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DOI: https://doi.org/10.1385/1-59259-754-8:001
Publisher Name: Humana Press
Print ISBN: 978-1-58829-329-9
Online ISBN: 978-1-59259-754-3
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