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Identification and Quantitation of G-Protein α-Subunits

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Receptor Signal Transduction Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 259))

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Abstract

The demonstration that many intracellular signaling processes are mediated by a family of closely related guanine nucleotide binding proteins (G-proteins) has led to the development of specific techniques that can be used to identify which of these polypeptide(s) is involved on receptor activation by ligand. In addition, these methods can be used to probe the specificity of the interaction and to yield information about the stoichiometries involved.

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© 2004 Humana Press Inc.

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Mullaney, I., Milligan, G. (2004). Identification and Quantitation of G-Protein α-Subunits. In: Willars, G.B., Challiss, R.A.J. (eds) Receptor Signal Transduction Protocols. Methods in Molecular Biology, vol 259. Humana Press. https://doi.org/10.1385/1-59259-754-8:207

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  • DOI: https://doi.org/10.1385/1-59259-754-8:207

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-329-9

  • Online ISBN: 978-1-59259-754-3

  • eBook Packages: Springer Protocols

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