Overview
- Editors:
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Mark J. Jaroszeski
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University of South Florida College of Medicine, USA
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Richard Heller
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University of South Florida College of Medicine, USA
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Richard Gilbert
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University of South Florida College of Engineering, USA
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Table of contents (35 protocols)
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Electrochemotherapy Protocols
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- Richard Gilbert, Mark J. Jaroszeski, Richard Heller
Pages 299-303
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- Stéphane Orlowski, Lluis M. Mir
Pages 305-311
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- Leif G. Salford, Per Engström, Bertil R. R. Persson
Pages 313-317
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- Mark J. Jaroszeski, Richard Gilbert, Richard Heller
Pages 319-326
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- Stéphane Orlowski, Lluis M. Mir
Pages 327-332
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Electrogenetherapy Protocols
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- Mark J. Jaroszeski, Richard Gilbert, Claude Nicolau, Richard Heller
Pages 333-338
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- Toru Nishi, Kimio Yoshizato, Tomoaki Goto, Hideo Takeshima, Shigeo Yamashiro, Jun-ichi Kuratsu et al.
Pages 339-348
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- Armand Keating, Edward Nolan, Robin Filshie, Sukhendu B. Dev
Pages 359-368
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Transdermal Delivery Protocols
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- Uwe Pliquett, Mark R. Prausnitz
Pages 377-406
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- Tani Chen, Robert Langer, James C. Weaver
Pages 407-436
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- Stephen A. Gallo, Patricia G. Johnson, Sek Wen Hui
Pages 437-445
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- Patricia G. Johnson, Stephen A. Gallo, Sek Wen Hui
Pages 447-455
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- Rita Vanbever, Véronique Préat
Pages 457-471
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Back Matter
Pages 489-497
About this book
Pulsed electric fields have been applied to living systems in vitro for a host of delivery applications since the early 1980s. It has been established that the primary effect that electrical treatment has on cells is an induced increase in the permeability of membranes to exogenous molecules. This state of increased permeability was noted to be temporary and could be induced with little or no effect on cell viability. This physical phenomenon was termed electroporation. Numerous published studies have shown that electroporation can be applied to any cell type. These studies also exploited the phenomenon to deliver drugs, DNA, antibodies, proteins, and fluorescent molecules. The use of electricity to mediate delivery of these molecule types in vitro has proven to be an invaluable research tool for biological and biomedical scientists. Many of the in vitro applications for electrically mediated delivery have tremendous potential for the treatment of human disease. For example, the ef- cient delivery of drugs and plasmid DNA has strong implications for improving standard therapies, as well as gene therapies. This potential was realized about 12 years ago when electric pulses were used to deliver drugs to tumor cells in vivo. Since then, the utility of in vivo electroporation for the delivery of m- ecules has been demonstrated through new applications that have been developed with increasing frequency each year. Electrochemotherapy, Electrogenetherapy, and Transdermal Drug Delivery: Electrically Mediated Delivery of Molecules to Cells provides review and protocol chapters that completely cover this relatively new scientific discipline.