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Part of the book series: Methods in Molecular Medicine ((MIMM,volume 37))

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Abstract

Electrochemotherapy (ECT) is a combination treatment which involves administering a chemotherapeutic agent followed by the delivery of electric pulses to cells or tissue. Electrical treatment results in increased drug uptake by the cells which provides an improved therapeutic benefit relative to using the drug alone. Increased drug uptake is due to a process that has been termed electropermeabilization, or electroporation.

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References

  1. Chang, D. C., Saunders, J. A., Chassy, B. M., and Sowers, A. E. (1992) Guide to Electroporation and Electrofusion. Academic Press, San Diego, CA.

    Google Scholar 

  2. Teissié, J. and Rols, M. P. (1992) Time course of electropermeabilization, in Charge and Field Effects in Biosystems, Vol. 3 (Allen, M. J., Cleary, S. F., Sowers, A. E., and Shillady, D. D., eds.), Birkhauser, Boston, MA, pp. 285–301.

    Google Scholar 

  3. Heller, R., Jaroszeski, M., Leo-Messina, J., Perrott, R., Van Voorhis, N., Reintgen, D., and Gilbert, R. (1995) Treatment of B16 melanoma with the combinations of electroporation and chemotherapy. Bioelectrochem. Bioenerg. 36, 83–87.

    Article  CAS  Google Scholar 

  4. Mir, L. M., Orlowski, S., Belehradek, J. Jr., and Paoletti, C. (1991) Electrochemotherapy potentiation of antitumour effect of bleomycin by local electric pulses. Eur. J. Cancer 27, 68–72.

    Article  PubMed  CAS  Google Scholar 

  5. Serša, G., Čemažar, M., and Miklavčič, D. (1995) Antitumor effectiveness of electrochemotherapy with cis-diamminedichloroplatinum (II) in mice. Cancer Res. 55, 3450–3455.

    PubMed  Google Scholar 

  6. Kanesada, H. (1990) Anticancer effect of high voltage pulses combined with concentration dependent anticancer drugs on lewis lung carcinoma, in vivo. J. Jpn. Soc. Cancer Ther. 25, 2640–2648.

    CAS  Google Scholar 

  7. Belehradek, J. Jr., Orlowski, S., Poddevin, B., Paoletti, C., and Mir, L. M. (1991) Electrochemotherapy of spontaneous mammary tumors in mice. Eur. J. Cancer 27, 73–76.

    Article  PubMed  Google Scholar 

  8. Okino, M., Tomie, H., Kanesada, H., Marumoto, M., Morita, N., Esato, K., and Suzuki, H. (1991) Induction of tumor specific selective toxicity in electrical impulse chemotherapy-analysis of dose response curve. Oncologia 24, 71–79.

    Google Scholar 

  9. Salford, L. G., Persson, B. R. R., Brun, A., Ceberg, C. P., Kongstad, P. C. H., and Mir, L. M. (1993) An new brain tumour therapy combining bleomycin with in vivo electropermeabilization. Biochem. Biophys. Res. Commun. 194, 938–943.

    Article  PubMed  CAS  Google Scholar 

  10. Heller, R., Jaroszeski, M. J., Glass, L. F., Messina, J. L., Rapaport, D. P., DeConti, R. C., Fenske, N. A., Gilbert, R. A., Mir, L. M., and Reintgen, D. S. (1996) Phase I/II trial for the treatment of cutaneous and subcutaneous tumors using electrochemotherapy. Cancer 77, 964–971.

    Article  PubMed  CAS  Google Scholar 

  11. Heller, R. (1995) Treatment of cutaneous nodules using electrochemotherapy. J. Florida Med. Assoc. 82, 147–150.

    CAS  Google Scholar 

  12. Rudolf, Z., Stabuc, B., Čemažar, M., Miklavčič, D. Vodovnik, L., and Serša, G. (1995) Electrochemotherapy with bleomycin: The first clinical experience in malignant melanoma patients. Radiol. Oncol. 29, 229–235.

    Google Scholar 

  13. Glass, L. F., Fenske, N. A., Jaroszeski, M. J., Perrott, R., Harvey, D. T., Reintgen D. S., and Heller, R. (1996) Bleomycin-mediated electrochemotherapy of basal cell carcinoma. J. Am. Acad. Dermatol. 34, 82–86.

    Article  PubMed  CAS  Google Scholar 

  14. Mir, L. M., Belehradek, M., Domenge, C., Orlowski, S., Poddevin, J. Jr., Schwab, G., Luboinnski, B., and Paoletti, C. (1991) Electrochemotherapy, a novel antitumor treatment: First clinical trial. C. R. Acad. Sci. 313, 613–618.

    CAS  Google Scholar 

  15. Belehradek, M., Domenge, C., Luboinnski, B., Orlowski, S., Belehradek, J., and Mir, L. M. (1993) Electrochemotherapy, a new anticancer treatment. Cancer 72, 694–3700.

    Article  Google Scholar 

  16. Domenge, C., Orlowski, S., Luboinski, B., DeBaere, T., Schwaab, G., Belehradek, Jr., and Mir, L. M. (1996) Antitumor electrochemotherapy. Cancer 77, 956–963.

    Article  PubMed  CAS  Google Scholar 

  17. Jaroszeski, M. J., Gilbert, R. A., and Heller, R. (1997) In vivo antitumor effects of lectrochemotherapy in a hepatoma model. Biochem. Biophys. Acta 1334, 15–18.

    PubMed  CAS  Google Scholar 

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© 2000 Humana Press Inc.

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Jaroszeski, M.J., Gilbert, R., Heller, R. (2000). Treatment of Liver Malignancies with Electrochemotherapy in a Rat Model. In: Jaroszeski, M.J., Heller, R., Gilbert, R. (eds) Electrochemotherapy, Electrogenetherapy, and Transdermal Drug Delivery. Methods in Molecular Medicine, vol 37. Humana Press. https://doi.org/10.1385/1-59259-080-2:319

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  • DOI: https://doi.org/10.1385/1-59259-080-2:319

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-606-2

  • Online ISBN: 978-1-59259-080-3

  • eBook Packages: Springer Protocols

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