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Basic Cloning Procedures

  • Book
  • © 1998

Overview

Editors:
  1. Valdis Berzins

    1. Biomedical Research and Study Centre, University of Latvia, Riga, Latvia

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  • Basic laboratory techniques, approved in several international molecular biology courses
  • Step-by-step protocols with detailed practical hints, troubleshooting guide and short background information
  • The protocols are written by authors with intensive practical experience

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (8 chapters)

  1. Front Matter

    Pages i-vii
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  2. cDNA Synthesis and Cloning

    • Juris Steinbergs, Aleksander Tsimanis
    Pages 1-21

  3. DNA Sequencing

    • Eriks Jankevics
    Pages 22-42

  4. RNA In Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase

    • Elita Avota, Normunds Licis
    Pages 43-52

  5. PCR-Based Site-Specific Mutagenesis

    • Janis Klovins, Valdis Berzins
    Pages 53-67

  6. Analysis of Specific Protein-DNA Interactions

    • Indrikis Muiznieks, Nils Rostoks
    Pages 68-103

  7. Gel Electrophoresis, Transfer and Hybridization of Nucleic Acids

    • Illar Pata, Erkki Truve
    Pages 104-134

  8. Immunological Methods for Analysis of Recombinant Proteins

    • Irina Sominskaya, Kaspars Tars
    Pages 135-144

  9. Protein Synthesis in Cell-Free Systems

    • Tiina Tamm, Erkki Truve
    Pages 145-163

  10. Back Matter

    Pages 165-169
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Keywords

About this book

This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.

Editors and Affiliations

  • Biomedical Research and Study Centre, University of Latvia, Riga, Latvia

    Valdis Berzins

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