Abstract
The chemical degradation method (Maxam and Gilbert 1977) and the dideoxy termination method (Sanger et al. 1977) are the basic techniques for sequencing DNA. Both of these methods depend on analytical polyacrylamide gel electrophoresis to resolve oligonucleotides with one identical end and one end varying in length by a single nucleotide. The enzymatic method has been improved over recent years. The Sanger method was developed as a basic method for large-scale DNA sequencing projects, using double-stranded DNA templates and the T7 DNA polymerase as a sequencing enzyme. A number of new modified enzymatic sequencing methods were developed recently: cycle sequencing, solid-phase sequencing, automated sequencing. In contrast, the original Maxam-Gilbert method has not been widely used for sequencing long DNA fragments.
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© 1998 Springer-Verlag Berlin Heidelberg
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Jankevics, E. (1998). DNA Sequencing. In: Berzins, V. (eds) Basic Cloning Procedures. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71965-3_2
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DOI: https://doi.org/10.1007/978-3-642-71965-3_2
Publisher Name: Springer, Berlin, Heidelberg
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