RNA In Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase

  • Elita Avota
  • Normunds Licis
Part of the Springer Lab Manual book series (SLM)

Abstract

The purpose of this chapter is to introduce beginners in molecular biology to RNA transcription by phage T7 DNA-dependent RNA polymerase. The work outlined here includes the transcription procedure of plasmid vectors or PCR-amplified DNA templates, the purification and identification of RNA products by sequencing with reverse transcriptase.

Keywords

Cellulose Phenol Urea Electrophoresis Polypeptide 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Elita Avota
    • 1
  • Normunds Licis
  1. 1.Biomedical Research and Study CentreUniversity of LatviaRigaLatvia

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