RNA In Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase

  • Elita Avota
  • Normunds Licis
Part of the Springer Lab Manual book series (SLM)


The purpose of this chapter is to introduce beginners in molecular biology to RNA transcription by phage T7 DNA-dependent RNA polymerase. The work outlined here includes the transcription procedure of plasmid vectors or PCR-amplified DNA templates, the purification and identification of RNA products by sequencing with reverse transcriptase.


Bacteriophage Promoter Extension Inhibition Transcription Vector Secondary Structure Effect Electrophoretic Concentrator 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Elita Avota
    • 1
  • Normunds Licis
  1. 1.Biomedical Research and Study CentreUniversity of LatviaRigaLatvia

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