RNA In Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase
The purpose of this chapter is to introduce beginners in molecular biology to RNA transcription by phage T7 DNA-dependent RNA polymerase. The work outlined here includes the transcription procedure of plasmid vectors or PCR-amplified DNA templates, the purification and identification of RNA products by sequencing with reverse transcriptase.
KeywordsCellulose Phenol Urea Electrophoresis Polypeptide
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