Gel Electrophoresis, Transfer and Hybridization of Nucleic Acids

  • Illar Pata
  • Erkki Truve
Part of the Springer Lab Manual book series (SLM)

Abstract

Gel electrophoresis is the basic tool for size fractionation of nucleic acids. The method has unparalleled resolving power and is performed with considerable ease and speed. In an electric field nucleic acids move through the pores in the gel towards the anode according to their molecular weight. After electrophoresis, the location of nucleic acids within the gel can be directly visualized in ultraviolet light by a dye ethidium bromide. If desired, these bands may be excised from the gel, nucleic acids purified and used in various manipulations (e.g. cloning, labeling). Furthermore, it is possible to identify a particular sequence in a composite population of nucleic acids by means of a blotting technique. The nucleic acids are separated by gel electrophoresis and then transferred from the gel to a filter so that their relative positions are preserved. The fragments of interest are identified by hybridization with a complementary nucleic acid probe.

Keywords

Formaldehyde Microwave Amide Aldehyde Diethyl 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Illar Pata
    • 1
  • Erkki Truve
    • 2
  1. 1.University of TartuTartuEstonia
  2. 2.Institute of Chemical Physics & BiophysicsTallinnEstonia

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