Skeletal Development and Repair

Methods and Protocols

  • Matthew J. Hilton

Part of the Methods in Molecular Biology book series (MIMB, volume 1130)

Table of contents

  1. Front Matter
    Pages i-x
  2. Understanding Mouse Skeletal Development and Repair

    1. Front Matter
      Pages 1-1
    2. Tatsuya Kobayashi, Henry M. Kronenberg
      Pages 3-12
    3. Elise F. Morgan, Anthony De Giacomo, Louis C. Gerstenfeld
      Pages 13-31
  3. Skeletal Repair, Transplantations, and Organ Cultures

    1. Front Matter
      Pages 33-33
    2. Anthony De Giacomo, Elise F. Morgan, Louis C. Gerstenfeld
      Pages 35-44
    3. Robinder S. Dhillon, Longze Zhang, Edward M. Schwarz, Brendan F. Boyce, Chao Xie
      Pages 45-59
    4. Daisuke Hamada, Erik R. Sampson, Robert D. Maynard, Michael J. Zuscik
      Pages 61-72
    5. Sys Hasslund, Regis J. O’Keefe, Hani A. Awad
      Pages 73-88
    6. Manuela Wuelling, Andrea Vortkamp
      Pages 89-97
    7. Rana Abou-Khalil, Céline Colnot
      Pages 99-110
  4. Histological and Molecular Analyses on Whole Tissues and Tissue Sections

    1. Front Matter
      Pages 111-111
    2. Diana Rigueur, Karen M. Lyons
      Pages 113-121
    3. Thomas B. Bemenderfer, Jonathan S. Harris, Keith W. Condon, Melissa A. Kacena
      Pages 123-147
    4. Sarah A. Mack, Kathleen M. Maltby, Matthew J. Hilton
      Pages 165-183
    5. X. L. Han, Jian Q. Feng
      Pages 185-191
    6. Timothy Rutkowsky, Deepika Sharma, Matthew J. Hilton
      Pages 193-201
    7. Yulia Shwartz, Elazar Zelzer
      Pages 203-215

About this book


Skeletal Development and Repair: Methods and Protocols is a compilation of a variety of skeletal research protocols utilizing the laboratory mouse as the platform for surgical manipulation and/or transplantation, as well as, the source of tissues and cells for in vitro culture and analyses. Chapters are written by experts in the field and cover topics including surgical, transplantation, and organ culture methods that permit analyses of skeletal tissues undergoing repair in vivo and permits analyses of cellular interactions ex vivo, histological and molecular techniques developed to study gene and protein expression in whole embryos, skeletal tissues and tissue sections, and in vitro primary cell culture protocols designed to assay gene function in specific cell populations. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Skeletal Development and Repair: Methods and Protocols is a comprehensive laboratory manual for all levels of basic research scientists working in the broad fields of skeletal development and skeletal repair research


cell assays skeletal development skeletal repair skeletal staining skeletal tissue

Editors and affiliations

  • Matthew J. Hilton
    • 1
  1. 1.Department of Orthopedics and RehabilitaUniversity of Rochester Medical CenterRochesterUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2014
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-988-8
  • Online ISBN 978-1-62703-989-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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