Abstract
The lacZ gene, encoding for the β-galactosidase enzyme, is widely used as a reporter gene in bone biology due to the ease of visualization in situ on whole-mount or on tissue sections. In this protocol we provide detailed methods for visualizing this reporter gene for both in vivo and in vitro studies. In addition, we describe a few tips for troubleshooting for mineralized tissues such as the skeleton.
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Han, X.L., Feng, J.Q. (2014). Beta-Galactosidase Staining in the Skeleton. In: Hilton, M. (eds) Skeletal Development and Repair. Methods in Molecular Biology, vol 1130. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-989-5_13
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DOI: https://doi.org/10.1007/978-1-62703-989-5_13
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-988-8
Online ISBN: 978-1-62703-989-5
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