Abstract
Whole-mount in situ hybridization (WISH) is a highly sensitive method for spatial and temporal visualization of RNA transcripts throughout an entire tissue. This method is an excellent tool for studying both embryonic development and disease. It is based on sequence complementation between a Digoxigenin (DIG)-labeled antisense RNA probe and the sense RNA transcript of interest in the tissue. The labeled probe is then visualized by immunohistochemical methods using an antibody conjugated to alkaline phosphatase that catalyzes a color reaction.
An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-62703-989-5_25
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Acknowledgements
This work was in part supported by the following NIH grants: R01 grants (AR057022 and AR063071), R21 grant (AR059733), T32 training grant (AR053459), and P30 Core Center grant (AR061307).
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Rutkowsky, T., Sharma, D., Hilton, M.J. (2014). Whole-Mount In Situ Hybridization on Murine Skeletogenic Tissues. In: Hilton, M. (eds) Skeletal Development and Repair. Methods in Molecular Biology, vol 1130. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-989-5_14
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DOI: https://doi.org/10.1007/978-1-62703-989-5_14
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