Overview
- Details fundamental issues and new applications of lipases and phospholipases
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 861)
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Table of contents (30 protocols)
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CLONING, PRODUCTION, PURIFICATION AND CHARACTERIZATION OF LIPASES AND PHOSPHOLIPASES
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OPTIMIZATION OF THE BIOCATALYST AND REACTION CONDITIONS
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APPLICATIONS OF LIPASES AND PHOPHOLIPASES
Keywords
About this book
Lipases are the most applied enzymes in organic synthesis due to their broad substrate acceptance and because of the availability of the molecular, biochemical, themodynamical and solvent engineering tools, which allows the optimization of lipases and lipase-catalyzed reactions. On the other hand, phospholipases are emerging as useful enzymes in food and pharmaceutical industries. In Lipases and Phospholipases: Methods and Protocols, expert researchers in the field provide key techniques to investigate these essential enzymes. Focusing on fundamental issues, current and new applications as well as practical step-by-step protocols, and the extensive applications of lipases and the potential application of phospholipases and its inhibitors. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.
 Thorough and intuitive, Lipases and Phospholipases: Methods and Protocols aids scientists in continuing to study lipases, phopholipases and related enzymes.
Editors and Affiliations
Bibliographic Information
Book Title: Lipases and Phospholipases
Book Subtitle: Methods and Protocols
Editors: Georgina Sandoval
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-600-5
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2012
Hardcover ISBN: 978-1-61779-599-2Published: 17 March 2012
Softcover ISBN: 978-1-4939-5877-1Published: 23 August 2016
eBook ISBN: 978-1-61779-600-5Published: 17 March 2012
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XV, 547
Number of Illustrations: 83 b/w illustrations, 18 illustrations in colour
Topics: Biochemistry, general, Enzymology
Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma