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Meiosis

Volume 2, Cytological Methods

  • Scott Keeney

Part of the Methods in Molecular Biology book series (MIMB, volume 558)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Cytological Analysis of Meiotic Chromosomes in Fungi

  3. Cytological Analysis of Meiotic Chromosomes in Plants and Small Animals

    1. Front Matter
      Pages 129-129
    2. Susan J. Armstrong, Eugenio Sanchez-Moran, F. Chris, H. Franklin
      Pages 131-145
    3. Carolyn M. Phillips, Kent L. McDonald, Abby F. Dernburg
      Pages 171-195
    4. Kim S. McKim, Eric F. Joyce, Janet K. Jang
      Pages 197-216
  4. Cytological and Histological Analysis of Mammalian Germ Cells and Meiotic Chromosomes

    1. Front Matter
      Pages 261-261
    2. Emad A. Ahmed, Dirk G. de Rooij
      Pages 263-277
    3. Sophie La Salle, Fengyun Sun, Mary Ann Handel
      Pages 279-297
    4. Martha Susiarjo, Carmen Rubio, Patricia Hunt
      Pages 339-354
    5. Esther de Boer, Franck G.P. Lhuissier, Christa Heyting
      Pages 355-382
    6. Evelyn Ko, Renée H. Martin
      Pages 401-418
    7. Ignasi Roig, Montserrat Garcia-Caldés
      Pages 419-431
    8. Shantha K. Mahadevaiah, Yael Costa, James M. A. Turner
      Pages 433-444
  5. Back Matter
    Pages 445-456

About this book

Introduction

The unique chromosome dynamics of meiosis have fascinated scientists for well over a century, but in recent years there has been a proliferation of new data concerning the pairing, recombination, and segregation of chromosomes. In Meiosis, expert researchers explore recent advances in three main areas: quantitative genetic methods for analysis of homologous recombination and chromosome pairing, development of direct physical assays for DNA intermediates and products of recombination, and cytological methods for characterizing chromosome behaviors and the patterns by which specific proteins associate with meiotic chromosomes. Meiosis: Volume 2, Cytological Methods, details methods for preparing and examining meiotic tissues, cells, and chromosomes by electron microscopy, indirect immunofluorescence, fluorescent in situ hybridization, and live-cell imaging. The methods in this volume are grouped for convenience by general types of organisms: fungi, plants and small animals (mostly invertebrates), and mammals. Composed in the highly successful Methods in Molecular Biology(tm) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Meiosis: Volume 2, Cytological Methods is an essential guidebook, providing up-to-date and critical new protocols for the study of meiotic chromosome dynamics.

Keywords

Chromosome dynamics in yeast DNA Drosophila Fluorescent in situ hybridization Indirect immunofluorescence Live-cell imaging Telomere Vertebrate electron microscopy fungi gene expression genes molecular biology proteins saccharomyces cerevisiae

Editors and affiliations

  • Scott Keeney
    • 1
  1. 1.Molecular Biology ProgramMemorial Sloan-Kettering Cancer CenterNew YorkU.S.A.

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60761-103-5
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-102-8
  • Online ISBN 978-1-60761-103-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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