Book Volume 1548 2017

Antimicrobial Peptides

Methods and Protocols

Editors:

ISBN: 978-1-4939-6735-3 (Print) 978-1-4939-6737-7 (Online)

Table of contents (32 protocols)

previous Page of 2
  1. Front Matter

    Pages i-xiv

  2. Properties, Design, Synthesis and Characterization of AMPs

    1. Front Matter

      Pages 1-1

    2. No Access

      Protocol

      Pages 3-22

      Antimicrobial Peptides: An Introduction

    3. No Access

      Protocol

      Pages 23-34

      Tools for Designing Amphipathic Helical Antimicrobial Peptides

    4. No Access

      Protocol

      Pages 35-49

      Chemical Synthesis of Antimicrobial Peptides

    5. No Access

      Protocol

      Pages 51-59

      Microwave-Assisted Synthesis of Antimicrobial Peptides

    6. No Access

      Protocol

      Pages 61-71

      High-Performance Liquid Chromatography and Mass Spectrometry-Based Design of Proteolytically Stable Antimicrobial Peptides

    7. No Access

      Protocol

      Pages 73-88

      Determination of Structure and Micellar Interactions of Small Antimicrobial Peptides by Solution-State NMR

    8. No Access

      Protocol

      Pages 89-99

      Mass Spectrometry Approaches for Determining the Structure of Antimicrobial Peptides

  3. Studying Interactions of AMPs with Model Membranes and Bacteria

    1. Front Matter

      Pages 101-101

    2. No Access

      Protocol

      Pages 103-118

      Molecular Dynamics Simulation and Analysis of the Antimicrobial Peptide–Lipid Bilayer Interactions

    3. No Access

      Protocol

      Pages 119-140

      Calorimetry Methods to Study Membrane Interactions and Perturbations Induced by Antimicrobial Host Defense Peptides

    4. No Access

      Protocol

      Pages 141-157

      Fluorescence and Absorbance Spectroscopy Methods to Study Membrane Perturbations by Antimicrobial Host Defense Peptides

    5. No Access

      Protocol

      Pages 159-180

      Applying Fluorescence Correlation Spectroscopy to Investigate Peptide-Induced Membrane Disruption

    6. No Access

      Protocol

      Pages 181-190

      Macromolecule Biosynthesis Assay and Fluorescence Spectroscopy Methods to Explore Antimicrobial Peptide Mode(s) of Action

    7. No Access

      Protocol

      Pages 191-199

      Using Confocal Microscopy and Computational Modeling to Investigate the Cell-Penetrating Properties of Antimicrobial Peptides

    8. No Access

      Protocol

      Pages 201-215

      Atomic Force Microscopy Study of the Interactions of Indolicidin with Model Membranes and DNA

    9. No Access

      Protocol

      Pages 217-230

      Protocols for Studying the Interaction of MSI-78 with the Membranes of Whole Gram-Positive and Gram-Negative Bacteria by NMR

    10. No Access

      Protocol

      Pages 231-245

      Preparation of Membrane Models of Gram-Negative Bacteria and Their Interaction with Antimicrobial Peptides Studied by CD and NMR

    11. No Access

      Protocol

      Pages 247-253

      Studying the Interaction of Magainin 2 and Cecropin A with E. coli Bacterial Cells Using Circular Dichroism

    12. No Access

      Protocol

      Pages 255-269

      Studying the Mechanism of Membrane Permeabilization Induced by Antimicrobial Peptides Using Patch-Clamp Techniques

    13. No Access

      Protocol

      Pages 271-281

      Assays for Identifying Inducers of the Antimicrobial Peptide LL-37

    14. No Access

      Protocol

      Pages 283-295

      Methods for Elucidating the Mechanism of Action of Proline-Rich and Other Non-lytic Antimicrobial Peptides

previous Page of 2