Abstract
Antimicrobial peptides (AMPs) may display the ability to penetrate cells, which may be relevant for their antibiotic activity. To investigate the relevance of the penetrating activity for the antibiotic activity of AMPs, here we describe a method based on the combined use of confocal microscopy and computational modeling coupled with cell death kinetics.
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Acknowledgment
We would like to thank Dr. Maria Teresa Lara Ortiz for her technical assistance in the writing of this work. Grants from the Alexander von Humboldt Foundation (FKZ: 3.4-IP-DEU/113263) and PAPIIT (IN205911) to GDR supported this work.
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Del Rio, G., Klipp, E., Herrmann, A. (2017). Using Confocal Microscopy and Computational Modeling to Investigate the Cell-Penetrating Properties of Antimicrobial Peptides. In: Hansen, P. (eds) Antimicrobial Peptides. Methods in Molecular Biology, vol 1548. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6737-7_13
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DOI: https://doi.org/10.1007/978-1-4939-6737-7_13
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