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Studying the Interaction of Magainin 2 and Cecropin A with E. coli Bacterial Cells Using Circular Dichroism

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Antimicrobial Peptides

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1548))

Abstract

The potential of antimicrobial peptides (AMPs) as an effective therapeutic alternative to classic and current antibiotics has encouraged studies to understand how they interact with the bacterial membrane. Here we describe how to detect, by circular dichroism (CD), the secondary structures of two antimicrobial peptides, magainin 2 and cecropin A, in the presence of E. coli bacterial cells.

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Author information

Authors and Affiliations

  1. Institute of Biostructure and Bioimaging CNR, via Mezzocannnone 16, 80134, Naples, Italy

    Concetta Avitabile, Luca Domenico D’Andrea & Alessandra Romanelli

  2. Department of Pharmacy, University of Naples “Federico II”, via Mezzocannone 16, Naples, 80134, Italy

    Alessandra Romanelli

Authors
  1. Concetta Avitabile
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  2. Luca Domenico D’Andrea
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  3. Alessandra Romanelli
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Corresponding author

Correspondence to Alessandra Romanelli .

Editor information

Editors and Affiliations

  1. Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

    Paul R. Hansen

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Avitabile, C., D’Andrea, L.D., Romanelli, A. (2017). Studying the Interaction of Magainin 2 and Cecropin A with E. coli Bacterial Cells Using Circular Dichroism. In: Hansen, P. (eds) Antimicrobial Peptides. Methods in Molecular Biology, vol 1548. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6737-7_17

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  • DOI: https://doi.org/10.1007/978-1-4939-6737-7_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6735-3

  • Online ISBN: 978-1-4939-6737-7

  • eBook Packages: Springer Protocols

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