Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1681)
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Table of contents (22 protocols)
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Key Basics of Phage Biology
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Sequencing Analysis of Bacteriophages
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Phage-Related Bioinformatics Tools
Keywords
About this book
This book expands on the previous volumes with new chapters exploring emerging themes and methodologies in bacterial virus research. The chapters in this book are divided into 4 parts and cover topics such as: iron chloride flocculation of bacteriophages from seawater; encapsulation of Listeria phage A511 by alginate; examining genome termini of bacteriophage through high-throughput sequencing; genome sequencing of dsDNA-containing bacteriophages directly from a single plaque; characterizing bacteriophages by biology, taxonomy, and genome analysis; phage genome annotation using the RAST pipeline; and the use of RP4::mini-Mu for gene transfer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and authoritative, Bacteriophages: Methods and Protocols, Volume IIIÂ is a valuable resource for both established and novice phage scientists.
Editors and Affiliations
Bibliographic Information
Book Title: Bacteriophages
Book Subtitle: Methods and Protocols, Volume 3
Editors: Martha R.J. Clokie, Andrew M. Kropinski, Rob Lavigne
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-7343-9
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2018
Hardcover ISBN: 978-1-4939-7341-5Published: 15 November 2017
Softcover ISBN: 978-1-4939-8458-9Published: 23 August 2018
eBook ISBN: 978-1-4939-7343-9Published: 10 November 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 316
Number of Illustrations: 25 b/w illustrations, 36 illustrations in colour
Topics: Microbiology
Industry Sectors: Biotechnology, Chemical Manufacturing, Consumer Packaged Goods, Pharma