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Practical Advice on the One-Step Growth Curve

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Bacteriophages

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1681))

Abstract

The one-step growth experiment is fundamental to the description of a new bacteriophage. The following protocol is optimized for those working with rapidly growing bacterial cultures.

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References

  1. Ellis EL, Delbrück M (1939) The growth of bacteriophage. J Gen Physiol 22:365–384

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  3. Symonds ND (1968) Experiment 14 – One-step growth curve and the Doermann experiment. In: Clowes RC, Hayes W (eds) Experiments in microbial genetics. Blackwell, Oxford, pp 75–78

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  4. Kelln RA, Warren RA (1971) Isolation and properties of a bacteriophage lytic for a wide range of pseudomonads. Can J Microbiol 17:677–682

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Author information

Authors and Affiliations

  1. Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, ON, Canada, N1G 2W1

    Andrew M. Kropinski

  2. Department of Pathobiology, University of Guelph, 50 Stone Road East, Guelph, ON, Canada, N1G 2W1

    Andrew M. Kropinski

  3. Department of Food Science, University of Guelph, 50 Stone Road East, Guelph, ON, Canada, N1G 2W1

    Andrew M. Kropinski

Authors
  1. Andrew M. Kropinski
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Corresponding author

Correspondence to Andrew M. Kropinski .

Editor information

Editors and Affiliations

  1. Department Infection, Immunity & Inflammation, University of Leicester School of Medicine, Leicester, United Kingdom

    Martha R.J. Clokie

  2. University of Guelph, Guelph, Ontario, Canada

    Andrew M. Kropinski

  3. KU Leuven, Laboratory of Gene Technology, Leuven, Belgium

    Rob Lavigne

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Kropinski, A.M. (2018). Practical Advice on the One-Step Growth Curve. In: Clokie, M., Kropinski, A., Lavigne, R. (eds) Bacteriophages. Methods in Molecular Biology, vol 1681. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7343-9_3

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  • DOI: https://doi.org/10.1007/978-1-4939-7343-9_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7341-5

  • Online ISBN: 978-1-4939-7343-9

  • eBook Packages: Springer Protocols

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