Glycobiology Protocols

  • Inka Brockhausen

Part of the Methods in Molecular Biology book series (MIMB, volume 347)

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Tamao Endo, Hiroshi Manya
    Pages 43-56
  3. Aleksandra Nita-Lazar, Robert S. Haltiwanger
    Pages 57-68
  4. Johannis P. Kamerling, Gerrit J. Gerwig
    Pages 69-91
  5. Silke Schrader, Roland Schauer
    Pages 93-107
  6. Catherine Robbe, Jean-Claude Michalski, Calliope Capon
    Pages 109-123
  7. Anne Imberty, Michaela Wimmerová, Jaroslav Koča, Christelle Breton
    Pages 145-156
  8. Fabio Dall’Olio, Nadia Malagolini, Mariella Chiricolo
    Pages 157-170
  9. Juan J. García-Vallejo, Sonja I. Gringhuis, Willem van Dijk, Irma van Die
    Pages 187-209
  10. Inka Brockhausen, Xiaojing Yang, Mark Harrison
    Pages 211-236
  11. Cristina L. Marolda, Piya Lahiry, Enrique Vinés, Soledad Saldías, Miguel A. Valvano
    Pages 237-252
  12. Inka Brockhausen, Walter A. Szarek, John G. Riley, Jason Z. Vlahakis
    Pages 253-265
  13. Frederick W. K. Kan
    Pages 289-304

About this book

Introduction

Glycobiology involves studies of complex carbohydrates and posttrans- tional modifications of proteins, and has become an important interdiscip- nary field encompassing chemistry, biochemistry, biology, physiology, and pathology. Although initial research was directed toward elucidation of the different carbohydrate structures and the enzymes synthesizing them, the field has now moved toward identifying the functions of carbohydrates. The pro- cols described in Glycobiology Protocols form a solid basis for investigations of glycan functions in health and disease. The cloning of many of the genes participating in glycosylation processes has helped to enhance our knowledge of how glycosylation is controlled, but has also added another dimension of complexity to the great heterogeneous variety of the structures of the oligos- charides of glycoproteins, proteoglycans, and glycolipids. A family of similar enzyme proteins exists for each glycosylation step. Glycosyltransferases are extremely specific for both the nucleotide sugar donor and the acceptor s- strate, but many other factors control sugar transfer, including the locali- tion and topology of enzymes, cofactors, possible chaperone proteins, and the availability of sugar acceptor substrates. The analysis of the intracellular organization of glycosylation and of the factors controlling the activities of the participating enzymes in the cell are important areas that need more research efforts. Another challenge for future research is to understand the glycodynamics of a cell, that is, how the cell responds to stimuli leading to biological and pathological changes in terms of alterations in glycosylation, and how this affects the biology of the cell.

Keywords

Lipid Oligosaccharid Polysaccharid biochemistry enzymes gene expression physiology proteins

Editors and affiliations

  • Inka Brockhausen
    • 1
    • 2
  1. 1.Department of MedicineQueen’s UniversityKingstonCanada
  2. 2.Department of BiochemistryQueen’s UniversityKingstonCanada

Bibliographic information

  • DOI https://doi.org/10.1385/1597451673
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-553-8
  • Online ISBN 978-1-59745-167-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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