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Structural Analysis of Naturally Occurring Sialic Acids

  • Johannis P. Kamerling
  • Gerrit J. Gerwig
Part of the Methods in Molecular Biology book series (MIMB, volume 347)

Abstract

Over the years several methodologies have been developed for the structural analysis of naturally occurring sialic acids (Sias), a family with more than 62 members. Currently there are two primary instrumental approaches: analysis of volatile Sia derivatives by gas-liquid chromatography (GLC) combined with electron-impact mass spectrometry (EI/MS), and analysis of fluorescently labeled Sias by high-performance liquid chromatography (HPLC) eventually coupled with electrospray mass spectrometry (ESI/MS). This chapter presents both approaches in detail. The volatile Sia derivatives are comprised of trimethylsilylated methyl ester derivatives, heptafluorobutylated methyl ester derivatives, or pertrimethylsilylated derivatives. The fluorescent Sia derivatives are prepared by reaction with 1,2-diamino-4,5-methylenedioxybenzene. For the identification of the different Sia derivatives, detailed GLC, HPLC, EI/MS, and ESI/MS data are included.

Keywords

Sialic Acid Propionic Acid Neuraminic Acid Methyl Ester Derivative Collisional Activation Decomposition 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Johannis P. Kamerling
    • 1
  • Gerrit J. Gerwig
    • 1
  1. 1.Bijvoet Center, Department of Bio-Organic ChemistryUtrecht UniversityUtrechtThe Netherlands

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