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Partial Purification of Mannosylphosphorylundecaprenol Synthase From Micrococcus luteus

A Useful Enzyme for the Biosynthesis of a Variety of Mannosylphosphorylpolyisoprenol Products
  • Jeffrey S. Rush
  • Charles J. Waechter
Part of the Methods in Molecular Biology book series (MIMB, volume 347)

Abstract

Membrane fractions from Micrococcus luteus catalyze the transfer of mannose from GDP-mannose to mono- and dimannosyldiacylglycerol, mannosylphosphorylundecaprenol (Man-P-Undec), and a membrane-associated lipomannan. This chapter describes the detergent solubilization, partial purification, and properties of Man-P-Undec synthase. The mobility of the mannosyltransferase activity on sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicates that the enzyme is a polypeptide with a molecular weight of approx 30.7 kDa. Utilizing the broad specificity of the bacterial mannosyltransferase provides a useful approach for the enzymatic synthesis of a wide variety of Man-P-polyisoprenol products.

Keywords

Closed Circle Partial Purification Luria Broth Micrococcus Luteus Crude Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Jeffrey S. Rush
    • 1
  • Charles J. Waechter
    • 1
  1. 1.Department of Molecular and Cellular BiochemistryUniversity of Kentucky College of MedicineLexington

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