Partial Purification of Mannosylphosphorylundecaprenol Synthase From Micrococcus luteus

A Useful Enzyme for the Biosynthesis of a Variety of Mannosylphosphorylpolyisoprenol Products
  • Jeffrey S. Rush
  • Charles J. Waechter
Part of the Methods in Molecular Biology book series (MIMB, volume 347)


Membrane fractions from Micrococcus luteus catalyze the transfer of mannose from GDP-mannose to mono- and dimannosyldiacylglycerol, mannosylphosphorylundecaprenol (Man-P-Undec), and a membrane-associated lipomannan. This chapter describes the detergent solubilization, partial purification, and properties of Man-P-Undec synthase. The mobility of the mannosyltransferase activity on sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicates that the enzyme is a polypeptide with a molecular weight of approx 30.7 kDa. Utilizing the broad specificity of the bacterial mannosyltransferase provides a useful approach for the enzymatic synthesis of a wide variety of Man-P-polyisoprenol products.


Closed Circle Partial Purification Luria Broth Micrococcus Luteus Crude Membrane 
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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Jeffrey S. Rush
    • 1
  • Charles J. Waechter
    • 1
  1. 1.Department of Molecular and Cellular BiochemistryUniversity of Kentucky College of MedicineLexington

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