Gene Expression Profiling

Methods and Protocols

  • Richard A. Shimkets

Part of the Methods in Molecular Biology book series (MIMB, volume 258)

Table of contents

  1. Front Matter
    Pages i-x
  2. Richard A. Shimkets
    Pages 7-12
  3. James C. Willey, Erin L. Crawford, Charles R. Knight, K. A. Warner, Cheryl A. Motten, Elizabeth A. Herness et al.
    Pages 13-41
  4. Richard A. Shimkets
    Pages 43-51
  5. Marilyn C. Olson, Tsetska Takova, LuAnne Chehak, Michelle L. Curtis, Sarah M. Olson, Robert W. Kwiatkowski
    Pages 53-69
  6. Jennifer Lescallett, Marina E. Chicurel, Robert Lipshutz, Dennise D. Dalma-Weiszhausz
    Pages 71-94
  7. Zhijian J. Chen, Kenneth D. Tew
    Pages 95-106
  8. Denis V. Rebrikov, Sejal M. Desai, Paul D. Siebert, Sergey A. Lukyanov
    Pages 107-134
  9. Catheline Vilain, Gilbert Vassart
    Pages 135-152
  10. Martin Leach
    Pages 153-165
  11. Back Matter
    Pages 167-168

About this book

Introduction

The transcription of messenger RNA from a DNA template is a key process in a wide variety of biological systems. In Gene Expression Profiling: Methods and Protocols, leaders in gene expression methodology and bioinformatics data analysis share their best methods for measuring RNA levels in cells and tissues. Each proven protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Special care has been taken to distill the basic underlying principles of each method to a few straightforward concepts so that investigators can easily choose the method most appropriate to their application. The readily reproducible techniques presented include new methods for applying the GeneChip®, SAR-SAGE, StaRT-PCR, SSH, the Invader Assay®, and ADGEM. The authors also provide critical bioinformatics insight and resources for data analysis and management.
Cutting-edge and highly practical, Gene Expression Profiling: Methods and Protocols allows novice and experienced investigators alike not only to choose the best method to determine how genes are turned on and off in many different organisms, but also to use informatics resources to analyze and understand the results.

Editors and affiliations

  • Richard A. Shimkets
    • 1
  1. 1.CuraGen CorporationNew Haven

Bibliographic information

  • DOI https://doi.org/10.1385/1592597513
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-220-9
  • Online ISBN 978-1-59259-751-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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