Abstract
Scientists routinely lecture and write about gene expression and the abundance of transcripts, but in reality, they extrapolate this information from a variety of measurements that different technologies may provide. Indeed, there are many reasons that applying different technologies to transcript abundance may give different results. This may result from an incomplete understanding of the gene in question or from shortcomings in the applications of the technologies.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Reference
Ushkaryov, Y. A. and Sudhof, T. C. (1993) Neurexin IIIα: extensive alternative splicing generates membrane-bound and soluble forms. Proc. Natl. Acad. Sci. USA 90, 6410–6414.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2004 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Shimkets, R.A. (2004). Technical Considerations in Quantitating Gene Expression. In: Shimkets, R.A. (eds) Gene Expression Profiling. Methods in Molecular Biology, vol 258. Humana Press. https://doi.org/10.1385/1-59259-751-3:1
Download citation
DOI: https://doi.org/10.1385/1-59259-751-3:1
Publisher Name: Humana Press
Print ISBN: 978-1-58829-220-9
Online ISBN: 978-1-59259-751-2
eBook Packages: Springer Protocols