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Technical Considerations in Quantitating Gene Expression

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Gene Expression Profiling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 258))

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Abstract

Scientists routinely lecture and write about gene expression and the abundance of transcripts, but in reality, they extrapolate this information from a variety of measurements that different technologies may provide. Indeed, there are many reasons that applying different technologies to transcript abundance may give different results. This may result from an incomplete understanding of the gene in question or from shortcomings in the applications of the technologies.

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Reference

  1. Ushkaryov, Y. A. and Sudhof, T. C. (1993) Neurexin IIIα: extensive alternative splicing generates membrane-bound and soluble forms. Proc. Natl. Acad. Sci. USA 90, 6410–6414.

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© 2004 Humana Press Inc., Totowa, NJ

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Shimkets, R.A. (2004). Technical Considerations in Quantitating Gene Expression. In: Shimkets, R.A. (eds) Gene Expression Profiling. Methods in Molecular Biology, vol 258. Humana Press. https://doi.org/10.1385/1-59259-751-3:1

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  • DOI: https://doi.org/10.1385/1-59259-751-3:1

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-220-9

  • Online ISBN: 978-1-59259-751-2

  • eBook Packages: Springer Protocols

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