Cytokines and Colony Stimulating Factors

Methods and Protocols

  • Dieter Körholz
  • Wieland Kiess

Part of the Methods in Molecular Biology™ book series (MIMB, volume 215)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Introduction

  3. Detection Assays for Cytokines and Growth Factors

    1. Front Matter
      Pages 12-133
    2. Ursula Banning, Dieter Körholz
      Pages 21-22
    3. Jean Krutmann, Renz Mang, Susanne Grether-Beck
      Pages 23-27
    4. Bodo Hoffmeister, Felix Kiecker, Ingolf Surel, Elham Khatamzas, Volker Schuster, Hans-Dieter Volk et al.
      Pages 59-70
    5. Thomas Lehrnbecher, Stephen J. Chanock
      Pages 71-94
    6. Angelika Eggert, Hauke Sieverts, Henning Walczak
      Pages 95-116
    7. Angelika Eggert, John H. Maris
      Pages 117-133
  4. Detection Assays for Cytokines and Growth Factors

    1. Front Matter
      Pages 137-289
    2. Christof M. Kramm, Tim Niehues, Nikolai G. Rainov
      Pages 137-152
    3. Ciriaco A. Piccirillo, Gérald J. Prud’homme
      Pages 153-170
    4. Yuti Chernajovsky, Hanna Dreja, Kostas Triantaphyllopoulos, David Gould
      Pages 171-180
    5. Tohru Sakai, Kunisuke Himeno
      Pages 181-191
    6. Arne von Bonin, Solveig H. Moré, Minka Breloer
      Pages 193-200

About this book


Enormous effort has been spent developing many new laboratory techniques for studying immune cells and the immune system, not only to increase fundamental understanding, but also to procure therapeutic success in a clinical setting. In Cytokines and Colony Stimulating Factors: Methods and Protocols, leading laboratory experts describe for the novice investigator a host of novel technologies and molecular techniques specifically designed to study cellular immunology and promote its gene therapy applications. Presented in step-by-step detail to ensure ready reproducibility, these protocols range from flow cytometric techniques to detect cytokines and growth factors in different specimens, to methods for generating and expanding dendritic cells and hematopoietic progenitors from different origins. Each proven method has been fine-tuned for experimental successs by the investigator who developed it, and includes notes on troubleshooting and avoiding potential pitfalls. Although the protocols require little previous experience, even highly skilled researchers will augment their experimental range with many novel timesaving techniques.
Cutting-edge and highly practical, Cytokines and Colony Stimulating Factors: Methods and Protocols provides researchers a carefully evaluated set of powerful tools for successful experimental research on cytokines and their role in gene therapy.

Editors and affiliations

  • Dieter Körholz
    • 1
  • Wieland Kiess
    • 1
  1. 1.Department of PediatricsUniversity of LeipzigLeipzigGermany

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-035-9
  • Online ISBN 978-1-59259-345-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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