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Flow-Cytometric Immune Function Methodology for Human Peripheral Blood Dendritic Cells

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Cytokines and Colony Stimulating Factors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 215))

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References

  1. Willmann, K. and Dunne, J. F. (2000) A flow cytometric immune function assay for human peripheral blood dendritic cells. J. Leuk. Biol. 67, 536–544.

    Article  CAS  Google Scholar 

  2. Olweus, J., BitMansour, A., Warnke, R., Thompson, P. A., Carballido, J., Picker, L. J., et al. (1997) Dendritic cell ontogeny: a human dendritic cell lineage of myeloid origin. Proc. Natl. Acad. Sci. USA 94, 12,551–12,556.

    Article  CAS  Google Scholar 

  3. O’Doherty, U., Peng, M., Gezelter, S., Swiggard, W. J., Betjes, M., Bhardwaj, N., et al. (1994) Human blood contains two subsets of dendritic cells, one immunologi-cally mature and the other immature. Immunology 82, 487–493.

    CAS  Google Scholar 

  4. Liu, Y-J. and Blom, B. (2000) Focus on hematology. Introduction: TH2-inducing DC2 for immunotherapy. Blood 95, 2482–2483.

    Article  CAS  Google Scholar 

  5. Shapiro, H. M., ed. (1995) Practical Flow Cytometry. Wiley-Liss, New York.

    Google Scholar 

  6. Givan, A. L., ed. (1992) Flow Cytometry First Principles. Wiley-Liss, New York.

    Google Scholar 

  7. Young, N. S. and Beris, P. (2001) Flow cytometry in hematology and oncology. Semin. Hematol. 38, 93–200.

    Google Scholar 

  8. Stein, D. S., Korvick, J. A., and Vermund S. H. (1992) CD4+lymphocyte cell enumeration for prediction of clinical course of human immunodeficiency virus disease: a review. J. Infect. Dis. 166, 1198–1199.

    Article  Google Scholar 

  9. Maecker, H. T., Maino, V. C., and Picker, L. J. (2000) Immunofluorescence analysis of T-cell responses in health and disease. J. Clin. Immunol. 20, 391–399.

    Article  CAS  Google Scholar 

  10. Karanikas, V., Lodding, J., Maino, V. C., and McKenzie, I. F. C. (2000) Flow cytometric measurement of intracellular cytokines detects immune responses in MUC1 immunotherapy. Clin. Cancer Res. 6, 829–837.

    CAS  Google Scholar 

  11. Bleesing, J. J. H. and Fleisher, T. A. (2001) Cell function-based flow cytometry. Semin. Hematol. 38, 169–178.

    Article  CAS  Google Scholar 

  12. Picker, L. J. and Maino, V. C. (2000) The CD4+T cell resonse to HIV-1. Curr. Opin. Immunol. 12, 381–386.

    Article  CAS  Google Scholar 

  13. Auffermann-Gretzinger, S., Keeffe, E.., and Levy, S. (2001) Impaired dendritic cell maturation in patients with chronic, but not resolved, hepatitis C virus infection. Blood 97, 3171–3176.

    Article  CAS  Google Scholar 

  14. Steinman, R. M. (1996) The dendritic cell in clinical immunology: the AIDS example. J. Lab. Clin. Med. 128, 531–535.

    Article  CAS  Google Scholar 

  15. Nouri-Shirazi, M., Banchereau, J., Fay, J., and Palucka, K. (2000) Dendritic cell based tumor vaccines. Immunol. Lett. 74, 5–10.

    Article  CAS  Google Scholar 

  16. Vuckovic, S., Fearnley, D. B., Gunningham, S., Spearing, R. L., Patton, W. N., and Hart, D. N. J. (1999) Dendritic cells in chronic myelomonocytic leukaemia. Br. J. Hematol. 105, 974–985.

    Article  CAS  Google Scholar 

  17. Hart, D. N. J. (1997) Dendritic cells: unique leukocyte populations which control the primary immune response. Blood 90, 3245–3287.

    Article  CAS  Google Scholar 

  18. Kapsenberg, M. L. Hilkens C. M. U., Wierenga, E. A., and Kalinski, P. (1999) The paradigm of type 1 and type 2 antigen-presenting cells. Implications for atopic allergy. Clin. Exp. Allergy 29, 33–36.

    Article  Google Scholar 

  19. Pulendran, B. Maraskovsky, E., Banchereau, J., and Maliszewski, C. (2001) Review. Modulating the immune response with dendritic cells and their growth factors. Trends Immunol. 22, 41–47.

    Article  CAS  Google Scholar 

  20. Sato, M., Iwakaba, K., Kimura, S., and Nishimura, T. (1998) Functional skewing of bone marrow-derived dendritic cells by Th1-or Th2-inducing cytokines. Immunol. Lett. 67, 63–68.

    Article  Google Scholar 

  21. Ghanekar, S., Zheng, L., Logar, A., Navratil, J., Borowski, L., Gupta, P., et al. (1996) Cytokine expression by human peripheral blood dendritic cells stimulated in vitro with HIV-1 and herpes simplex virus. J. Immunol. 157, 4028–4036.

    CAS  Google Scholar 

  22. Kohrgruber, N., Halanek, N., Gröger, M., Winter, D., Rappersberger, K., Schmitt-Egenolf, M., et al. (1999) Survival, maturation, and function of CD11c-and CD11c+peripheral blood dendritic cells are differentially regulated by cytokines. J. Immunol. 163, 3250–3259.

    CAS  Google Scholar 

  23. Banchereau, J. and Steinman, R. M. (1998) Dendritic cells and the control of immunity. Nature 392, 245–252.

    Article  CAS  Google Scholar 

  24. Lanzavecchia, A. and Sallusto, F. (2000) Dynamics of T lymphocyte responses: intermediates, effectors, and memory cells. Science 290, 92–97.

    Article  CAS  Google Scholar 

  25. Hart, D. N. J., Clark, G. J., Dekker, J. W., Fearnley, D. B., Kato, M., Hock, B. D., et al. (1997) Dendritic cell surface molecules. Dendritic Cells Fundam.Clin. Immunol. 439–442.

    Google Scholar 

  26. Banchereau, J., Pulendran, B., Steinman, R., and Palucka, K. (2000) Commentary. Will the making of plasmacytoid dendritic cells in vitro help unravel their mysteries? J. Exp. Med. 192, F39–F44.

    Article  CAS  Google Scholar 

  27. Rissoan, M.-C., Soumelis, V., Kadowaki, N., Grouard, G., Briere, F., de Waal Malefyt, R., et al. (1999) Report. Reciprocal control of T helper cell and dendritic cell differentiation. Science 283, 1183–1186.

    Article  CAS  Google Scholar 

  28. Arpinati, M., Green, C. L., Heimfeld, S., Heuser, J. E., and Anasetti, C. (2000) Focus on hematology. Granulocyte-colony stimulating factor mobilizes T helper 2-inducing dendritic cells. Blood 95, 2484–2490.

    Article  CAS  Google Scholar 

  29. Heufler, C., Koch, F., Stanzl, U., Topar, G., Wysocka, M., Trinchieri, G., et al. (1996) Interleukin-12 is produced by dendritic cells and mediates T helper 1 development as well as interferon-γ production by T helper 1 cells. Eur. J. Immunol. 26, 659–668.

    Article  CAS  Google Scholar 

  30. Lapointe, R., Toso, J. F., Butts, H. A., Young, H. A., and Hwu, P. (2000) Human dendritic cells require multiple activation signals for the efficient generation of tumor antigen-specific T lymphocytes. Eur. J. Immunol. 30, 3291–3298.

    Article  CAS  Google Scholar 

  31. Zhou, L.-J. and Tedder, T. F. (1995) A distinct pattern of cytokine gene expression by human CD83+blood dendritic cells. Blood 86, 3295–3301.

    Article  CAS  Google Scholar 

  32. Nomura, L. E., Walker, J. M., and Maecker, H. T. (2000) Optimization of whole blood antigen-specific cytokine assays for CD4+T cells. Cytometry 40, 60–68.

    Article  CAS  Google Scholar 

  33. Suni, M. A., Picker, L. J., and Maino, V. C. (1998) Detection of antigen-specific T cell cytokine expression in whole blood by flow cytometry. J. Immunol. Methods 212, 89–98.

    Article  CAS  Google Scholar 

  34. Waldrop, S. L., Pitcher, C. J., Peterson, D. M., Maino, V. C., and Picker, L. J. (1997) Determination of antigen-specific memory/effector CD4+T cell frequencies by flow cytometry. J. Clin. Invest. 99, 1739–1750.

    Article  CAS  Google Scholar 

  35. Jung, T., Schauer, U., Heusser, C., Neumann, C., and Rieger, C. (1993) Detection of intracellular cytokines by flow cytometry. J. Immunol. Methods 159, 197–207.

    Article  CAS  Google Scholar 

  36. Picker, L. J., Singh, M. K., Zdraveski, Z., Treer, J. R., Waldrop, S. L., Bergstresser, P. R., et al. (1995) Direct demonstration of cytokine synthesis heterogeneity among human memory/effector T cells by flow cytometry. Blood 86, 1408–1419.

    Article  CAS  Google Scholar 

  37. De Caestecker, M. P., Telfer, B. A., Hutchinson, I. V., and Ballardie, E W. (1992) The detection of intracytoplasmic interleukin-1α, interleukin-β, an tumour necrosis factor a expression in human monocytes using two colour immunofluorescence flow cytometry. J. Immunol Methods 154, 11–20.

    Article  CAS  Google Scholar 

  38. Zhou, L.-J. and Tedder, T. E (1995) Human blood dendritic cells selectively express CD83, a member of the immunoglobulin superfamily. J. Immunol. 154 3821–3835.

    CAS  Google Scholar 

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Willmann, K. (2003). Flow-Cytometric Immune Function Methodology for Human Peripheral Blood Dendritic Cells. In: Körholz, D., Kiess, W. (eds) Cytokines and Colony Stimulating Factors. Methods in Molecular Biology, vol 215. Humana, Totowa, NJ. https://doi.org/10.1385/1-59259-345-3:41

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  • DOI: https://doi.org/10.1385/1-59259-345-3:41

  • Publisher Name: Humana, Totowa, NJ

  • Print ISBN: 978-1-58829-035-9

  • Online ISBN: 978-1-59259-345-3

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