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Glycoprotein Methods and Protocols

The Mucins

  • Anthony P. Corfield

Part of the Methods in Molecular Biology™ book series (MIMB, volume 125)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Purification of Mucin

    1. Front Matter
      Pages 1-1
    2. Julia R. Davies, Ingemar Carlstedt
      Pages 3-13
    3. Kermit L. Carraway
      Pages 15-26
  3. Detection and Ouantitation of Mucin

    1. Front Matter
      Pages 27-27
    2. Michael D. Walsh, Jeremy R. Jass
      Pages 29-44
    3. Adrian Allen, Jeffrey P. Pearson
      Pages 57-64
    4. Jan Dekker, B. Van Jan-Willem Klinken, Hans A. Büller, Alexandra W. C. Einerhand
      Pages 65-73
  4. Separation, Identification, and Physical Characterization of Mucins

    1. Front Matter
      Pages 75-75
    2. David J. Thornton, Nagma Khan, John K. Sheehan
      Pages 77-85
    3. John K. Sheehan, David J. Thornton
      Pages 87-96
  5. Rheology of Mucin

    1. Front Matter
      Pages 97-97
    2. Jeffrey P. Pearson, Adrian Allen, David A. Hutton
      Pages 99-109
  6. Mucin Peptide Analysis

    1. Front Matter
      Pages 111-111
    2. Jun X. Yan, Nicolle H. Packer
      Pages 113-119
    3. Natasha E. Zachara, Andrew A. Gooley
      Pages 121-128
    4. Andrea Murray, Deirdre A. O’Sullivan, Michael R. Price
      Pages 129-141
    5. Sherilyn L. Bell, Janet F. Forstner
      Pages 143-155
  7. Carbohydrate Structural Analysis of Mucins

    1. Front Matter
      Pages 157-157
    2. Jean-Claude Michalski, Calliope Capon
      Pages 159-180
    3. Elizabeth F. Hounsell
      Pages 181-190
    4. André Klein, Gérard Strecker, Geneviève Lamblin, Philippe Roussel
      Pages 191-209
    5. Mathew J. Harrison, Nicolle H. Packer
      Pages 211-216
  8. Mucin Biosynthesis

    1. Front Matter
      Pages 217-217
    2. Anthony P. Corfield, Neil Myerscough, Alexandra W. C. Einerhand, B. Van Jan-Willem Klinken, Jan Dekker, Christos Paraskeva
      Pages 219-226
    3. Anthony P. Corfield, Neil Myerscough, B. Van Jan-Willem Klinken, Alexandra W. C. Einerhand, Jan Dekker
      Pages 227-237
    4. B. Van Jan-Willem Klinken, Hans A. Büller, Alexandra W. C. Einerhand, Jan Dekker
      Pages 239-247
    5. Alexandra W. C. Einerhand, B. Van Jan-Willem Klinken, Hans A. Buller, Jan Dekker
      Pages 249-259
    6. Guillemette Huet, Philippe Delannoy, Thécla Lesuffleur, Sylviane Hennebicq, Pierre Degand
      Pages 261-271
    7. Inka Brockhausen
      Pages 273-293
  9. Mucin Gene Detection

    1. Front Matter
      Pages 295-295
    2. Jean-Pierre Aubert, Nicole Porchet
      Pages 297-303
    3. Nicole Porchet, Jean-Pierre Aubert
      Pages 305-312
    4. Nicole Porchet, Jean-Pierre Aubert
      Pages 313-321
    5. Lynne E. Vinall, Wendy S. Pratt, Dallas M. Swallow
      Pages 337-350
  10. Preparation of Antimucin Antibodies

    1. Front Matter
      Pages 351-351
    2. Francisco X. Real, Carme de Bolós, Egbert Oosterwijk
      Pages 353-368
    3. Pei Xiang Xing, Vasso Apostolopoulos, Jim Karkaloutsos, Ian F. C. McKenzie
      Pages 369-381
  11. Enzymatic Degradation of Mucins

    1. Front Matter
      Pages 383-383
    2. Roger M. Stark, Rebecca Wiggins, Elizabeth Walley, Sally J. Hicks, Gulnaz A. Gill, Stephen D. Carrington et al.
      Pages 385-392
    3. David A. Hutton, Adrian Allen, Jeffrey P. Pearson
      Pages 393-401
    4. Anthony P. Corfield, Neil Myerscough
      Pages 403-416
    5. Anthony M. Roberton, Douglas I. Rosendale, Damian P. Wright
      Pages 417-426
  12. Mucin-Bacterial Interactions

    1. Front Matter
      Pages 427-427
    2. Nancy A. McNamara, Robert A. Sack, Suzanne M. J. Fleiszig
      Pages 429-437
    3. George T. Macfarlane, Sandra Macfarlane
      Pages 439-452
  13. Cellular And Humoral Responses To Mucins

    1. Front Matter
      Pages 453-453
    2. Vasso Apostolopoulos, Ian F. C. McKenzie, Geoffrey A. Pietersz
      Pages 455-462

About this book

Introduction

Anthony P. Corfield and a group of hands-on expermentalists bring together in Glycoprotein Methods and Protocols: The Mucins a state-of-the-art collection of reliable and tested methods for the study of all aspects of mucins. These powerful methods range from the preparation of mucins to their detection and quantitation with molecular biological and biochemical reagents. Also included are methods for the assessment of mucin peptide and carbohydrate, of mucin biosynthesis and degradation, and of mucin bacteriology and cell biology. The techniques take advantage of the latest improvements in sensitivity and specificity of detection, and in the preparation of new reagents for specific biochemical detection. Glycoprotein Methods and Protocols: The Mucins offers today's researchers a complete range of readily reproducible analytical techniques for studying mucins. Its powerful techniques constitute a launching pad for those entering the field for the first time and a state-of-the-art collection for those already active in mucin research.

Editors and affiliations

  • Anthony P. Corfield
    • 1
  1. 1.Bristol Royal InfirmaryBristolUK

Bibliographic information

  • DOI https://doi.org/10.1385/1592590489
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-720-5
  • Online ISBN 978-1-59259-048-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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