Glycoprotein Methods and Protocols

The Mucins

  • Anthony P. Corfield

Part of the Methods in Molecular Biology™ book series (MIMB, volume 125)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Purification of Mucin

    1. Front Matter
      Pages 1-1
    2. Julia R. Davies, Ingemar Carlstedt
      Pages 3-13
    3. Kermit L. Carraway
      Pages 15-26
  3. Detection and Ouantitation of Mucin

    1. Front Matter
      Pages 27-27
    2. Michael D. Walsh, Jeremy R. Jass
      Pages 29-44
    3. Adrian Allen, Jeffrey P. Pearson
      Pages 57-64
    4. Jan Dekker, B. Van Jan-Willem Klinken, Hans A. Büller, Alexandra W. C. Einerhand
      Pages 65-73
  4. Separation, Identification, and Physical Characterization of Mucins

    1. Front Matter
      Pages 75-75
    2. David J. Thornton, Nagma Khan, John K. Sheehan
      Pages 77-85
    3. John K. Sheehan, David J. Thornton
      Pages 87-96
  5. Rheology of Mucin

    1. Front Matter
      Pages 97-97
    2. Jeffrey P. Pearson, Adrian Allen, David A. Hutton
      Pages 99-109
  6. Mucin Peptide Analysis

    1. Front Matter
      Pages 111-111
    2. Jun X. Yan, Nicolle H. Packer
      Pages 113-119
    3. Natasha E. Zachara, Andrew A. Gooley
      Pages 121-128
    4. Andrea Murray, Deirdre A. O’Sullivan, Michael R. Price
      Pages 129-141
    5. Sherilyn L. Bell, Janet F. Forstner
      Pages 143-155
  7. Carbohydrate Structural Analysis of Mucins

    1. Front Matter
      Pages 157-157

About this book


Anthony P. Corfield and a group of hands-on expermentalists bring together in Glycoprotein Methods and Protocols: The Mucins a state-of-the-art collection of reliable and tested methods for the study of all aspects of mucins. These powerful methods range from the preparation of mucins to their detection and quantitation with molecular biological and biochemical reagents. Also included are methods for the assessment of mucin peptide and carbohydrate, of mucin biosynthesis and degradation, and of mucin bacteriology and cell biology. The techniques take advantage of the latest improvements in sensitivity and specificity of detection, and in the preparation of new reagents for specific biochemical detection. Glycoprotein Methods and Protocols: The Mucins offers today's researchers a complete range of readily reproducible analytical techniques for studying mucins. Its powerful techniques constitute a launching pad for those entering the field for the first time and a state-of-the-art collection for those already active in mucin research.

Editors and affiliations

  • Anthony P. Corfield
    • 1
  1. 1.Bristol Royal InfirmaryBristolUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-720-5
  • Online ISBN 978-1-59259-048-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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