Overview
- Editors:
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Anthony P. Corfield
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Bristol Royal Infirmary, Bristol, UK
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Table of contents (41 protocols)
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Enzymatic Degradation of Mucins
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Front Matter
Pages 383-383
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- Roger M. Stark, Rebecca Wiggins, Elizabeth Walley, Sally J. Hicks, Gulnaz A. Gill, Stephen D. Carrington et al.
Pages 385-392
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- David A. Hutton, Adrian Allen, Jeffrey P. Pearson
Pages 393-401
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- Anthony P. Corfield, Neil Myerscough
Pages 403-416
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- Anthony M. Roberton, Douglas I. Rosendale, Damian P. Wright
Pages 417-426
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Mucin-Bacterial Interactions
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Front Matter
Pages 427-427
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- Nancy A. McNamara, Robert A. Sack, Suzanne M. J. Fleiszig
Pages 429-437
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- George T. Macfarlane, Sandra Macfarlane
Pages 439-452
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Cellular And Humoral Responses To Mucins
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Front Matter
Pages 453-453
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- Vasso Apostolopoulos, Ian F. C. McKenzie, Geoffrey A. Pietersz
Pages 455-462
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- John R. McKolanis, Olivera J. Finn
Pages 463-470
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- Pawel Ciborowski, Olivera J. Finn
Pages 471-486
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- Joy Burchell, Joyce Taylor-Papadimitriou
Pages 487-493
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- Silvia von Mensdorff-Pouilly, Claus Vennegoor, Joseph Hilgers
Pages 495-500
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Back Matter
Pages 501-506
About this book
The mucins (mucus glycoproteins) have long been a complex corner of glycoprotein biology. While dramatic advances in the separation, structural an- ysis, biosynthesis, and degradation have marked the progress in general glycop- tein understanding, the mucins have lagged behind. The reasons for this lack of progress have always been clear and are only now being resolved. The mucins are very large molecules; they are difficult to separate from other molecules present in mucosal secretions or membranes; they are often degraded owing to natural protective functions or to isolation methodology and their peptide and oligos- charide structures are varied and complex. Understanding these molecules has demanded progress in several major areas. Isolation techniques that protect the intact mucins and allow dissociation from other adsorbed but discrete molecules needed to be developed and accepted by all researchers in the field. Improved methods for the study of very large molecules with regard to their aggregation and polymerization were also needed. Structural analysis of the peptide domains and the multitude of oligosaccharide chains was required for smaller sample sizes, for multiple samples, and in shorter time. In view of these problems it is perhaps not surprising that the mucins have remained a dilemma, of obvious biological importance and interest, but very difficult to analyze.
Reviews
"There is sufficient detail to follow easily and unlike many research papers, the minute details which make for success of failure, all appear to included." -Microbiology Today
Editors and Affiliations
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Bristol Royal Infirmary, Bristol, UK
Anthony P. Corfield